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A more recent version of this article appeared on February 1, 2004
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Submitted on June 6, 2003
Revised on September 5, 2003
Accepted on October 15, 2003
1 Institut de Biologie et Chimie des Protéines, UMR5086 - CNRS/Université Lyon I, IFR 128 BioSciences Lyon-Gerland, 7 Passage du Vercors, 69367 Lyon cedex 07, France
2 Department of Molecular Cell Research, Max-Planck-Institute for Medical Research, Jahnstrasse 29, D-69120 Heidelberg, Germany
3 Institute for Systems Biology, 4225 Roosevelt Way NE, Seattle WA 98105, USA
4 Université de Genève, Centre Médical Universitaire, Département de Morphologie, CH-1211 Genève 4, Switzerland
5 Laboratoire de Biochimie et Biophysique des Systémes Intégrés, 38054 Grenoble Cedex 9, France
6 Department of Biological Sciences, Imperial College of Science Technology and Medicine, London SW7 2AZ, United Kingdom
* Corresponding author. E-mail address: f.letourneur{at}ibcp.fr.
The best described function of the adaptor complex-1 (AP-1) is to participate in the budding of clathrin-coated vesicles from the trans-Golgi network and endosomes. Here we show that AP-1 is also localized to phagocytic cups in murine macrophages as well as in Dictyostelium amoebae. AP-1 is recruited to phagosomal membranes at this early stage of phagosome formation and rapidly dissociates from maturing phagosomes. To establish the role of AP-1 in phagocytosis, we made used of Dictyostelium mutant cells (apm1- cells) disrupted for AP-1 medium chain. In this mutant, phagocytosis drops by 60% indicating that AP-1 is necessary for efficient phagocytosis. Furthermore, phagocytosis in apm1- cells is more affected for large rather than small particles and cells exhibiting incomplete engulfment are then often observed. This suggests that AP-1 could participate in the extension of the phagocytic cup. Interestingly, macropinocytosis, a process dedicated to fluid-phase endocytosis and related to phagocytosis, is also impaired in apm1- cells. In summary, our data suggest a new role of AP-1 at an early stage of phagosome and macropinosome formation.
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