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A more recent version of this article appeared on February 1, 2004
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Submitted on June 8, 2003
Revised on October 17, 2003
Accepted on October 27, 2003
1 Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, 1 University Station A4800, The University of Texas at Austin, Austin, Texas 78712 USA
2 Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, 1 University Station A4800, The University of Texas at Austin, Austin, Texas 78712 USA, Present Address: Department of
Microbiology and Immunology, The University of Michigan Medical School,
6734 Medical Sciences Building II, Ann Arbor, Michigan 48109 USA
3 Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, 1 University Station A4800, The University of Texas at Austin, Austin, Texas 78712 USA, overnight mail address: The University of Texas at Austin, 2500 Speedway, Austin, Texas 78712 USA
* Corresponding author. E-mail address: jaf{at}mail.utexas.edu.
Photoreceptor nuclei in the Drosophila eye undergo developmentally regulated migrations. Nuclear migration is known to require the perinuclear protein Klarsicht, but the function of Klarsicht has been obscure. Here, we show that Klarsicht is required for connecting the microtubule organizing center (MTOC) to the nucleus. In addition, in a genetic screen for klarsicht-interacting genes, we identified Lam Dm0, which encodes nuclear lamin. We find that, like Klarsicht, lamin is required for photoreceptor nuclear migration and for nuclear attachment to the MTOC. Moreover, perinuclear localization of Klarsicht requires lamin. We propose that nuclear migration requires linkage of the MTOC to the nucleus through an interaction between microtubules, Klarsicht, and lamin. The Klarsicht/lamin interaction provides a framework for understanding the mechanistic basis of human laminopathies.
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