![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on February 1, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on June 24, 2003
Revised on October 13, 2003
Accepted on October 17, 2003
1 Department of Pathology, Stanford University School of Medicine, Stanford, California 94305, USA
2 Department of Urology, Stanford University School of Medicine, Stanford, California 94305, USA
3 Division of Nephrology, Stanford University School of Medicine, Stanford, California 94305, USA
4 Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California 94305, USA; Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305, USA
* Corresponding author. E-mail address: john.higgins{at}stanford.edu.
The kidney is a highly specialized organ with a complex, stereotyped architecture and a great diversity of functions and cell types. Because the microscopic organization of the nephron, the functional unit of the kidney, has a consistent relationship to the macroscopic anatomy of the kidney, knowledge of the characteristic patterns of gene expression in different compartments of the kidney could provide insight into the functions and functional organization of the normal nephron. We studied gene expression in dissected renal lobes of five adult human kidneys using cDNA microarrays representing 
30,000 different human genes. Total RNA was isolated from sections of the inner and outer cortex, inner and outer medulla, papillary tips, and renal pelvis and from glomeruli isolated by sieving. The results revealed unique and highly distinctive patterns of gene expression for glomeruli, cortex, medulla, papillary tips, and pelvic samples. Immunohistochemical staining using selected antisera confirmed differential expression of several cognate proteins and provided histological localization of expression within the nephron. The distinctive patterns of gene expression in discrete portions of the kidney may serve as a resource for further understanding of renal physiology and the molecular and cellular organization of the nephron.
This article has been cited by other articles:
|
|
 |
|
  L. He, Y. Sun, M. Takemoto, J. Norlin, K. Tryggvason, T. Samuelsson, and C. Betsholtz The Glomerular Transcriptome and a Predicted Protein-Protein Interaction Network J. Am. Soc. Nephrol., February 1, 2008; 19(2): 260 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Gruber, C. D. Coldren, M. D. Woolum, G. P. Cosgrove, C. Zeng, A. E. Baron, M. D. Moore, C. D. Cool, G. S. Worthen, K. K. Brown, et al. Human Lung Project: Evaluating Variance of Gene Expression in the Human Lung Am. J. Respir. Cell Mol. Biol., July 1, 2006; 35(1): 65 - 71. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Devarajan Update on Mechanisms of Ischemic Acute Kidney Injury J. Am. Soc. Nephrol., June 1, 2006; 17(6): 1503 - 1520. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Eikmans, J. A. Aben, K. Koop, H. J. Baelde, E. de Heer, and J. A. Bruijn Genetic factors in progressive renal disease: the good ones, the bad ones and the ugly ducklings Nephrol. Dial. Transplant., February 1, 2006; 21(2): 257 - 260. [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Storey, W. Xiao, J. T. Leek, R. G. Tompkins, and R. W. Davis Significance analysis of time course microarray experiments PNAS, September 6, 2005; 102(36): 12837 - 12842. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. O. Dowling and D. Bienzle Gene-expression changes induced by Feline immunodeficiency virus infection differ in epithelial cells and lymphocytes J. Gen. Virol., August 1, 2005; 86(8): 2239 - 2248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. Son, S. Bilke, S. Davis, B. T. Greer, J. S. Wei, C. C. Whiteford, Q.-R. Chen, N. Cenacchi, and J. Khan Database of mRNA gene expression profiles of multiple human organs Genome Res., March 1, 2005; 15(3): 443 - 450. [Abstract] [Full Text] [PDF]
|
|
