![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on December 1, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on June 25, 2003
Revised on July 31, 2003
Accepted on July 31, 2003
1 Dept. of Biol. Sciences, Carnegie Mellon University, Pittsburgh, PA 15213
* Corresponding author. E-mail address: linstedt{at}andrew.cmu.edu.
It is unclear whether the mammalian Golgi apparatus can form de novo from the ER or whether it requires a preassembled Golgi matrix. As a test, we assayed Golgi reassembly after forced redistribution of Golgi matrix proteins into the ER. Two conditions were used. In one, ER redistribution was achieved using a combination of BFA to cause Golgi collapse and H89 to block ER export. Unlike brefeldin A alone, which leaves matrix proteins in relatively large remnant structures outside the ER, the addition of H89 to BFA-treated cells caused ER accumulation of all Golgi markers tested. In the other, clofibrate treatment induced ER redistribution of matrix and nonmatrix proteins. Significantly, Golgi reassembly after either treatment was robust implying that the Golgi has the capacity to form de novo from the ER. Furthermore, matrix proteins reemerged from the ER with faster ER exit rates. This, together with the sensitivity of BFA remnants to ER export blockade, suggests that presence of matrix proteins in BFA remnants is due to cycling via the ER and preferential ER export rather than their stable assembly in a matrix outside the ER. In summary, the Golgi apparatus appears capable of efficient self-assembly.
This article has been cited by other articles:
|
|
 |
|
  H. Pan, J. Yu, L. Zhang, A. Carpenter, H. Zhu, L. Li, D. Ma, and J. Yuan A Novel Small Molecule Regulator of Guanine Nucleotide Exchange Activity of the ADP-ribosylation Factor and Golgi Membrane Trafficking J. Biol. Chem., November 7, 2008; 283(45): 31087 - 31096. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Gong, D. Sengupta, A. D. Linstedt, and R. Schwartz Simulated De Novo Assembly of Golgi Compartments by Selective Cargo Capture during Vesicle Budding and Targeted Vesicle Fusion Biophys. J., August 15, 2008; 95(4): 1674 - 1688. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-w. Lee, X. Zhao, Y.-I. Yim, E. Eisenberg, and L. E. Greene Essential Role of Cyclin-G-associated Kinase (Auxilin-2) in Developing and Mature Mice Mol. Biol. Cell, July 1, 2008; 19(7): 2766 - 2776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. N. Feinstein and A. D. Linstedt GRASP55 Regulates Golgi Ribbon Formation Mol. Biol. Cell, July 1, 2008; 19(7): 2696 - 2707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Guo, V. Punj, D. Sengupta, and A. D. Linstedt Coat-Tether Interaction in Golgi Organization Mol. Biol. Cell, July 1, 2008; 19(7): 2830 - 2843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wakana, S. Takai, K.-i. Nakajima, K. Tani, A. Yamamoto, P. Watson, D. J. Stephens, H.-P. Hauri, and M. Tagaya Bap31 Is an Itinerant Protein That Moves between the Peripheral Endoplasmic Reticulum (ER) and a Juxtanuclear Compartment Related to ER-associated Degradation Mol. Biol. Cell, May 1, 2008; 19(5): 1825 - 1836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Jollivet, G. Raposo, A. Dimitrov, R. Sougrat, B. Goud, and F. Perez Analysis of De Novo Golgi Complex Formation after Enzyme-based Inactivation Mol. Biol. Cell, November 1, 2007; 18(11): 4637 - 4647. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Langhans, C. Hawes, S. Hillmer, E. Hummel, and D. G. Robinson Golgi Regeneration after Brefeldin A Treatment in BY-2 Cells Entails Stack Enlargement and Cisternal Growth followed by Division Plant Physiology, October 1, 2007; 145(2): 527 - 538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Radulescu, A. Siddhanta, and D. Shields A Role for Clathrin in Reassembly of the Golgi Apparatus Mol. Biol. Cell, January 1, 2007; 18(1): 94 - 105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Bejarano, M. Cabrera, L. Vega, J. Hidalgo, and A. Velasco Golgi structural stability and biogenesis depend on associated PKA activity J. Cell Sci., September 15, 2006; 119(18): 3764 - 3775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jiang, S. W. Rhee, P. A. Gleeson, and B. Storrie Capacity of the Golgi Apparatus for Cargo Transport Prior to Complete Assembly Mol. Biol. Cell, September 1, 2006; 17(9): 4105 - 4117. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Guo and A. D. Linstedt COPII-Golgi protein interactions regulate COPII coat assembly and Golgi size J. Cell Biol., July 3, 2006; 174(1): 53 - 63. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. H. Ho, C. Y. He, C. L. de Graffenried, L. J. Murrells, and G. Warren Ordered assembly of the duplicating Golgi in Trypanosoma brucei PNAS, May 16, 2006; 103(20): 7676 - 7681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Steet and S. Kornfeld COG-7-deficient Human Fibroblasts Exhibit Altered Recycling of Golgi Proteins Mol. Biol. Cell, May 1, 2006; 17(5): 2312 - 2321. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Altan-Bonnet, R. Sougrat, W. Liu, E. L. Snapp, T. Ward, and J. Lippincott-Schwartz Golgi Inheritance in Mammalian Cells Is Mediated through Endoplasmic Reticulum Export Activities Mol. Biol. Cell, February 1, 2006; 17(2): 990 - 1005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Mardones, C. M. Snyder, and K. E. Howell Cis-Golgi Matrix Proteins Move Directly to Endoplasmic Reticulum Exit Sites by Association with Tubules Mol. Biol. Cell, January 1, 2006; 17(1): 525 - 538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Kondylis, K. M. Spoorendonk, and C. Rabouille dGRASP Localization and Function in the Early Exocytic Pathway in Drosophila S2 Cells Mol. Biol. Cell, September 1, 2005; 16(9): 4061 - 4072. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-d. Yang, R. Elamawi, J. Bubeck, R. Pepperkok, C. Ritzenthaler, and D. G. Robinson Dynamics of COPII Vesicles and the Golgi Apparatus in Cultured Nicotiana tabacum BY-2 Cells Provides Evidence for Transient Association of Golgi Stacks with Endoplasmic Reticulum Exit Sites PLANT CELL, May 1, 2005; 17(5): 1513 - 1531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kapetanovich, C. Baughman, and T. H. Lee Nm23H2 Facilitates Coat Protein Complex II Assembly and Endoplasmic Reticulum Export in Mammalian Cells Mol. Biol. Cell, February 1, 2005; 16(2): 835 - 848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Reinke, P. Kozik, and B. S. Glick Golgi inheritance in small buds of Saccharomyces cerevisiae is linked to endoplasmic reticulum inheritance PNAS, December 28, 2004; 101(52): 18018 - 18023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Natarajan and A. D. Linstedt A Cycling cis-Golgi Protein Mediates Endosome-to-Golgi Traffic Mol. Biol. Cell, November 1, 2004; 15(11): 4798 - 4806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Starkuviene, U. Liebel, J. C. Simpson, H. Erfle, A. Poustka, S. Wiemann, and R. Pepperkok High-Content Screening Microscopy Identifies Novel Proteins With a Putative Role in Secretory Membrane Traffic Genome Res., October 1, 2004; 14(10a): 1948 - 1956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Puri, H. Telfer, M. Velliste, R. F. Murphy, and A. D. Linstedt Dispersal of Golgi matrix proteins during mitotic Golgi disassembly J. Cell Sci., January 22, 2004; 117(3): 451 - 456. [Abstract] [Full Text] [PDF]
|
|
