![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on February 1, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on July 11, 2003
Revised on October 7, 2003
Accepted on October 17, 2003
1 Istituto di Genetica Molecolare. Consiglio Nazionale delle Ricerche. Via Ferrata 1. 27100 Pavia, Italy
* Corresponding author. E-mail address: biamonti{at}igm.cnr.it.
Heat shock triggers the assembly of nuclear stress bodies that contain Heat Shock Factor 1 and a subset of RNA processing factors. These structures are formed on the pericentromeric heterochromatic regions of specific human chromosomes, among which chromosome 9. In this paper we show that these heterochromatic domains are characterized by an epigenetic status typical of euchromatic regions. Similarly to transcriptionally competent portions of the genome, stress bodies are, in fact, enriched in acetylated histone H4. Acetylation peaks at 6 h of recovery from heat shock. Moreover, heterochromatin markers, such as HP1 and histone H3 methylated on lysine 9, are excluded from these nuclear districts. In addition, heat shock triggers the transient accumulation of RNA molecules, heterogeneous in size, containing the subclass of satellite III sequences found in the pericentromeric heterochromatin of chromosome 9. This is the first report of a transcriptional activation of a constitutive heterochromatic portion of the genome in response to stress stimuli.
This article has been cited by other articles:
|
|
 |
|
  R. Valgardsdottir, I. Chiodi, M. Giordano, A. Rossi, S. Bazzini, C. Ghigna, S. Riva, and G. Biamonti Transcription of Satellite III non-coding RNAs is a general stress response in human cells Nucleic Acids Res., February 2, 2008; 36(2): 423 - 434. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lu and D. M. Gilbert Proliferation-dependent and cell cycle regulated transcription of mouse pericentric heterochromatin J. Cell Biol., November 5, 2007; 179(3): 411 - 421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Carchilan, M. Delgado, T. Ribeiro, P. Costa-Nunes, A. Caperta, L. Morais-Cecilio, R. N. Jones, W. Viegas, and A. Houben Transcriptionally Active Heterochromatin in Rye B Chromosomes PLANT CELL, June 1, 2007; 19(6): 1738 - 1749. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Papait, C. Pistore, D. Negri, D. Pecoraro, L. Cantarini, and I. M. Bonapace Np95 Is Implicated in Pericentromeric Heterochromatin Replication and in Major Satellite Silencing Mol. Biol. Cell, March 1, 2007; 18(3): 1098 - 1106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. V. Prasanth and D. L. Spector Eukaryotic regulatory RNAs: an answer to the 'genome complexity' conundrum Genes & Dev., January 1, 2007; 21(1): 11 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Jolly and S. C. Lakhotia Human sat III and Drosophila hsr{omega} transcripts: a common paradigm for regulation of nuclear RNA processing in stressed cells Nucleic Acids Res., November 14, 2006; 34(19): 5508 - 5514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Bouzinba-Segard, A. Guais, and C. Francastel Accumulation of small murine minor satellite transcripts leads to impaired centromeric architecture and function PNAS, June 6, 2006; 103(23): 8709 - 8714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. K. Shumaker, T. Dechat, A. Kohlmaier, S. A. Adam, M. R. Bozovsky, M. R. Erdos, M. Eriksson, A. E. Goldman, S. Khuon, F. S. Collins, et al. From the Cover: Mutant nuclear lamin A leads to progressive alterations of epigenetic control in premature aging PNAS, June 6, 2006; 103(23): 8703 - 8708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. H. Fox, C. S. Bond, and A. I. Lamond P54nrb Forms a Heterodimer with PSP1 That Localizes to Paraspeckles in an RNA-dependent Manner Mol. Biol. Cell, November 1, 2005; 16(11): 5304 - 5315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Valgardsdottir, I. Chiodi, M. Giordano, F. Cobianchi, S. Riva, and G. Biamonti Structural and Functional Characterization of Noncoding Repetitive RNAs Transcribed in Stressed Human Cells Mol. Biol. Cell, June 1, 2005; 16(6): 2597 - 2604. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Metz, J. Soret, C. Vourc'h, J. Tazi, and C. Jolly A key role for stress-induced satellite III transcripts in the relocalization of splicing factors into nuclear stress granules J. Cell Sci., September 1, 2004; 117(19): 4551 - 4558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Chiodi, M. Corioni, M. Giordano, R. Valgardsdottir, C. Ghigna, F. Cobianchi, R.-M. Xu, S. Riva, and G. Biamonti RNA recognition motif 2 directs the recruitment of SF2/ASF to nuclear stress bodies Nucleic Acids Res., August 9, 2004; 32(14): 4127 - 4136. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jia, K.-i. Noma, and S. I. S. Grewal RNAi-Independent Heterochromatin Nucleation by the Stress-Activated ATF/CREB Family Proteins Science, June 25, 2004; 304(5679): 1971 - 1976. [Abstract] [Full Text] [PDF]
|
|
