Molecular Biology of the Cell track citations

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print December 2, 2003
Mol. Biol. Cell 10.1091/mbc.E03-08-0589

A more recent version of this article appeared on February 1, 2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E03-08-0589v1
15/2/801    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maùecki, J.
Right arrow Articles by Olsnes, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maùecki, J.
Right arrow Articles by Olsnes, S.

Submitted on August 13, 2003
Revised on October 3, 2003
Accepted on October 21, 2003

Translocation of FGF-1 and FGF-2 across vesicular membranes occurs during G1-phase by a common mechanism

Jædrzej Maùecki1, Jrgen Wesche1, Camilla Skiple Skjerpen1, Antoni Wiêdlocha1, and Sjur Olsnes1*

1 The Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway

* Corresponding author. E-mail address: olsnes{at}radium.uio.no.

The entry of exogenous fibroblast growth factor 2 (FGF-2) to the cytosolic/nuclear compartment was studied and compared with the translocation mechanism used by FGF-1. To differentiate between external and endogenous growth factor we used FGF-2 modified to contain a farnesylation signal, a CaaX-box. Since farnesylation occurs only in the cytosol and nucleoplasm, farnesylation of exogenous FGF-2-CaaX was taken as evidence that the growth factor had translocated across cellular membranes. We found that FGF-2 translocation occurred in endothelial cells and fibroblasts, which express FGF receptors, and that the efficiency of translocation was increased in the presence of heparin. Concomitantly with translocation, the 18 kDa FGF-2 was N-terminally cleaved to yield a 16 kDa form. Translocation of FGF-2 required PI3-kinase activity but not transport through the Golgi apparatus. Inhibition of endosomal acidification did not prevent translocation, whereas dissipation of the vesicular membrane potential completely blocked it. The data indicate that translocation occurs from intracellular vesicles containing proton pumps and that an electrical potential across the vesicle membrane is required. Translocation of both FGF-1 and FGF-2 occurred during most of G1 but decreased shortly before the G1->S transition. A common mechanism for FGF-1 and FGF-2 translocation into cells is postulated.




This article has been cited by other articles:


Home page
 BloodHome page
V. Stepanova, T. Lebedeva, A. Kuo, S. Yarovoi, S. Tkachuk, S. Zaitsev, K. Bdeir, I. Dumler, M. S. Marks, Y. Parfyonova, et al.
Nuclear translocation of urokinase-type plasminogen activator
Blood, July 1, 2008; 112(1): 100 - 110.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
V. Sorensen, Y. Zhen, M. Zakrzewska, E. M. Haugsten, S. Walchli, T. Nilsen, S. Olsnes, and A. Wiedlocha
Phosphorylation of Fibroblast Growth Factor (FGF) Receptor 1 at Ser777 by p38 Mitogen-Activated Protein Kinase Regulates Translocation of Exogenous FGF1 to the Cytosol and Nucleus
Mol. Cell. Biol., June 15, 2008; 28(12): 4129 - 4141.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Nilsen, K. R. Rosendal, V. Sorensen, J. Wesche, S. Olsnes, and A. Wiedlocha
A Nuclear Export Sequence Located on a beta-Strand in Fibroblast Growth Factor-1
J. Biol. Chem., September 7, 2007; 282(36): 26245 - 26256.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
V. Sorensen, A. Wiedlocha, E. M. Haugsten, D. Khnykin, J. Wesche, and S. Olsnes
Different abilities of the four FGFRs to mediate FGF-1 translocation are linked to differences in the receptor C-terminal tail
J. Cell Sci., October 15, 2006; 119(20): 4332 - 4341.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. A. Dupree, S. R. Pollack, E. M. Levine, and C. T. Laurencin
Fibroblast Growth Factor 2 Induced Proliferation in Osteoblasts and Bone Marrow Stromal Cells: A Whole Cell Model
Biophys. J., October 15, 2006; 91(8): 3097 - 3112.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Wesche, J. Malecki, A. Wiedlocha, C. S. Skjerpen, P. Claus, and S. Olsnes
FGF-1 and FGF-2 Require the Cytosolic Chaperone Hsp90 for Translocation into the Cytosol and the Cell Nucleus
J. Biol. Chem., April 21, 2006; 281(16): 11405 - 11412.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
A. Joliot
Transduction Peptides Within Naturally Occurring Proteins
Sci. Signal., December 6, 2005; 2005(313): pe54 - pe54.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
E. Millette, B. H. Rauch, O. Defawe, R. D. Kenagy, G. Daum, and A. W. Clowes
Platelet-Derived Growth Factor-BB-Induced Human Smooth Muscle Cell Proliferation Depends on Basic FGF Release and FGFR-1 Activation
Circ. Res., February 4, 2005; 96(2): 172 - 179.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Wiedlocha, T. Nilsen, J. Wesche, V. Sorensen, J. Malecki, E. Marcinkowska, and S. Olsnes
Phosphorylation-regulated Nucleocytoplasmic Trafficking of Internalized Fibroblast Growth Factor-1
Mol. Biol. Cell, February 1, 2005; 16(2): 794 - 810.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2003 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.