Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print May 28, 2004
Mol. Biol. Cell 10.1091/mbc.E03-09-0680

A more recent version of this article appeared on August 1, 2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E03-09-0680v1
15/8/3811    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gueldener, U.
Right arrow Articles by Hegemann, J. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gueldener, U.
Right arrow Articles by Hegemann, J. H.

Submitted on September 18, 2003
Revised on May 10, 2004
Accepted on May 16, 2004

Characterisation of the Saccharomyces cerevisiae Fol1 Protein: Starvation for C1 Carrier Induces Pseudohyphal Growth

Ulrich Gueldener*{dagger}{ddagger}, Gabriele J. Koehler*{ddagger}, Christoph Haussmann{sect}, Adelbert Bacher{sect}, Jörn Kricke||, Dietmar Becher¶, and Johannes H. Hegemann*#

*Funktionelle Genomforschung der Mikroorganismen, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany; {sect}Institut für Organische Chemie und Biochemie, Technische Universität München, 85747 Garching, Germany; ||AG Anatomie und Zellbiologie der Charite, 13353 Berlin, Germany; and MICROMUN Private Institute for Microbiological Research GmbH, Biotechnikum Greifswald, 17489 Greifswald, Germany

Monitoring Editor: Douglas Koshland

Tetrahydrofolate (vitamin B9) and its folate derivatives are essential cofactors in one-carbon (C1) transfer reactions and absolutely required for the synthesis of a variety of different compounds including methionine and purines. Most plants, microbial eukaryotes and prokaryotes synthesize folate de novo. We have characterized an important enzyme in this pathway, the S. cerevisiae FOL1 gene. Expression of the budding yeast gene FOL1 in E. coli identified the folate biosynthetic enzyme activities dihydroneopterin aldolase (DHNA), 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase (HPPK),and dihydropteroate synthase (DHPS). All 3 enzyme activities were also detected in wild-type yeast strains, while fol1{Delta} deletion strains only showed background activities thus demonstrating that Fol1p catalyzes three sequential steps of the tetrahydrofolate biosynthetic pathway and thus is the central enzyme of this pathway which starting from GTP consists of 7 enzymatic reactions in total. Fol1p is exclusively localized to mitochondria as shown by fluorescence microscopy and immune electron microscopy. FOL1 is an essential gene and the nongrowth phenotype of the fol1 deletion leads to a recessive auxotrophy for folinic acid (5'-formyltetrahydrofolate). Growth of the fol1{Delta} deletion strain on folinic acid supplemented rich media induced a dimorphic switch with haploid invasive and filamentous pseudohyphal growth in the presence of glucose and ammonium which are known suppressors of filamentous and invasive growth. The invasive growth phenotype induced by the depletion of C1 carrier is dependent on the transcription factor Ste12p and the flocullin/adhesin Flo11p, while the filamentation phenotype is independent of Ste12p, Tec1p, Phd1p and Flo11p suggesting other signaling pathways as well as other adhesion proteins.

{dagger}Present address: Institute of Bioinformatics, GSF - National Research Center for Environment and Health, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany

{ddagger}These authors contributed equally to this work

#Corresponding author.

Johannes H. Hegemann, E-mail: hegemann{at}uni-duesseldorf.de




This article has been cited by other articles:


Home page
 Eukaryot CellHome page
J. Botet, L. Mateos, J. L. Revuelta, and M. A. Santos
A Chemogenomic Screening of Sulfanilamide-Hypersensitive Saccharomyces cerevisiae Mutants Uncovers ABZ2, the Gene Encoding a Fungal Aminodeoxychorismate Lyase
Eukaryot. Cell, November 1, 2007; 6(11): 2102 - 2111.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Storozhenko, O. Navarrete, S. Ravanel, V. De Brouwer, P. Chaerle, G.-F. Zhang, O. Bastien, W. Lambert, F. Rebeille, and D. Van Der Straeten
Cytosolic Hydroxymethyldihydropterin Pyrophosphokinase/Dihydropteroate Synthase from Arabidopsis thaliana: A SPECIFIC ROLE IN EARLY DEVELOPMENT AND STRESS RESPONSE
J. Biol. Chem., April 6, 2007; 282(14): 10749 - 10761.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2004 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.