![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on March 1, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on September 19, 2003
Revised on October 30, 2003
Accepted on October 31, 2003
Adaptin Sorting Signal
1 Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201
2 Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201, Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI 48109
3 Department of Surgery, Wayne State University School of Medicine, Detroit, MI 48201
4 Department of Pharmacology, Department of Surgery, Wayne State University School of Medicine, Elliman Building, room 1205, 421 E. Canfield, Detroit, MI 48201
* Corresponding author. E-mail address: ndavis{at}genetics.wayne.edu.
Our previous work found the two yeast plasma membrane-localized casein kinases Yck1p and Yck2p to be palmitoylated on C-terminal Cys-Cys sequences by the palmitoyl transferase Akr1p. The present work examines a third casein kinase, Yck3p which ends with the C-terminal sequence Cys-Cys-Cys-Cys-Phe-Cys-Cys-Cys. Yck3p is palmitoylated and localized to the vacuolar membrane. While the C-terminal cysteines are required for this palmitoylation, Akr1p is not. Palmitoylation requires the C-terminal Yck3p residues 463-524, while information for vacuolar sorting maps to the 409-462 interval. Vacuolar sorting is disrupted in cis through deletion of the 409-462 sequences and in trans through mutation of the AP-3 adaptin complex; both cis- and trans-mutations result in Yck3p missorting to the plasma membrane. This missorted Yck3p restores 37°C viability to yck1
yck2-ts cells. yck1 yck2-ts suppressor mutations isolated within the YCK3 gene identify the Yck3p vacuolar sorting signal - the tetrapeptide YDSI, a perfect fit to the YXX
adaptin-binding consensus. While YXX signals have a well-appreciated role in the adaptin-mediated sorting of mammalian cells, this is the first signal of this class yet shown to operate in yeast.
This article has been cited by other articles:
|
|
 |
|
  N. Bharucha, J. Ma, C. J. Dobry, S. K. Lawson, Z. Yang, and A. Kumar Analysis of the Yeast Kinome Reveals a Network of Regulated Protein Localization during Filamentous Growth Mol. Biol. Cell, July 1, 2008; 19(7): 2708 - 2717. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Rubenstein and M. C. Schmidt Mechanisms Regulating the Protein Kinases of Saccharomyces cerevisiae Eukaryot. Cell, April 1, 2007; 6(4): 571 - 583. [Full Text] [PDF]
|
|
|
|
|
|
W. Wen, L. Chen, H. Wu, X. Sun, M. Zhang, and D. K. Banfield Identification of the Yeast R-SNARE Nyv1p as a Novel Longin Domain-containing Protein Mol. Biol. Cell, October 1, 2006; 17(10): 4282 - 4299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Yesilaltay, O. Kocher, R. Pal, A. Leiva, V. Quinones, A. Rigotti, and M. Krieger PDZK1 Is Required for Maintaining Hepatic Scavenger Receptor, Class B, Type I (SR-BI) Steady State Levels but Not Its Surface Localization or Function J. Biol. Chem., September 29, 2006; 281(39): 28975 - 28980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Mitchell, A. Vasudevan, M. E. Linder, and R. J. Deschenes Thematic review series: Lipid Posttranslational Modifications. Protein palmitoylation by a family of DHHC protein S-acyltransferases J. Lipid Res., June 1, 2006; 47(6): 1118 - 1127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. C. Hancock, R. P. Behta, and J. M. Lopes Genomic Analysis of the Opi- Phenotype Genetics, June 1, 2006; 173(2): 621 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Hou, K. Subramanian, T. J. LaGrassa, D. Markgraf, L. E. P. Dietrich, J. Urban, N. Decker, and C. Ungermann The DHHC protein Pfa3 affects vacuole-associated palmitoylation of the fusion factor Vac8 PNAS, November 29, 2005; 102(48): 17366 - 17371. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Y. Lee, K.-i. Taoka, B.-C. Yoo, G. Ben-Nissan, D.-J. Kim, and W. J. Lucas Plasmodesmal-Associated Protein Kinase in Tobacco and Arabidopsis Recognizes a Subset of Non-Cell-Autonomous Proteins PLANT CELL, October 1, 2005; 17(10): 2817 - 2831. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. G. Politis, A. F. Roth, and N. G. Davis Transmembrane Topology of the Protein Palmitoyl Transferase Akr1 J. Biol. Chem., March 18, 2005; 280(11): 10156 - 10163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. LaGrassa and C. Ungermann The vacuolar kinase Yck3 maintains organelle fragmentation by regulating the HOPS tethering complex J. Cell Biol., January 31, 2005; 168(3): 401 - 414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Babu, R. J. Deschenes, and L. C. Robinson Akr1p-dependent Palmitoylation of Yck2p Yeast Casein Kinase 1 Is Necessary and Sufficient for Plasma Membrane Targeting J. Biol. Chem., June 25, 2004; 279(26): 27138 - 27147. [Abstract] [Full Text] [PDF]
|
|
