Molecular Biology of the Cell Sign up for new MBC in Press e-TOCs!

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print March 26, 2004
Mol. Biol. Cell 10.1091/mbc.E03-10-0767

A more recent version of this article appeared on June 1, 2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E03-10-0767v1
15/6/2593    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Subramanian, S.
Right arrow Articles by Jones, E. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Subramanian, S.
Right arrow Articles by Jones, E. W.

Submitted on October 29, 2003
Revised on March 19, 2004
Accepted on March 19, 2004

The Sec1/Munc18 protein, Vps33p, functions at the endosome and the vacuole of Saccharomyces cerevisiae

Shoba Subramanian1*, Carol A. Woolford1, and Elizabeth W. Jones1

1 Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh PA 15213

* Corresponding author. E-mail address: shoba{at}andrew.cmu.edu.

The Sec1/Munc18 (SM) family of proteins is thought to impart compartmental specificity to vesicle fusion reactions. Here we report characterization of Vps33p, an SM family member previously thought to act exclusively at the vacuolar membrane with the vacuolar syntaxin Vam3p. Vacuolar morphology of vps33{Delta} cells resembles that of cells lacking both Vam3p and the endosomal syntaxin Pep12p, suggesting that Vps33p may function with these syntaxins at the vacuole and the endosome. Consistent with this, vps33 mutants secrete the Golgi precursor form of the vacuolar hydrolase CPY into the medium. We also demonstrate that Vps33p acts at other steps, for vps33 mutants show severe defects in endocytosis at the late endosome. At the endosome, Vps33p and other class C members exist as a complex with Vps8p, a protein previously known to act in transport between the late Golgi and the endosome. Vps33p also interacts with Pep12p, a known interactor of the SM protein Vps45p. High copy PEP7/VAC1 suppresses vacuolar morphology defects of vps33 mutants. These findings demonstrate that Vps33p functions at multiple trafficking steps and is not limited to action at the vacuolar membrane. This is the first report demonstrating the involvement of a single syntaxin with two SM proteins at the same organelle.




This article has been cited by other articles:


Home page
 MicrobiologyHome page
K. Franke, M. Nguyen, A. Hartl, H.-M. Dahse, G. Vogl, R. Wurzner, P. F. Zipfel, W. Kunkel, and R. Eck
The vesicle transport protein Vac1p is required for virulence of Candida albicans.
Microbiology, October 1, 2006; 152(Pt 10): 3111 - 3121.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. L. Grosshans, D. Ortiz, and P. Novick
Rabs and their effectors: Achieving specificity in membrane traffic
PNAS, August 8, 2006; 103(32): 11821 - 11827.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
C. Foote and S. F. Nothwehr
The clathrin adaptor complex 1 directly binds to a sorting signal in Ste13p to reduce the rate of its trafficking to the late endosome of yeast
J. Cell Biol., May 22, 2006; 173(4): 615 - 626.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
G. Wang and R. J. Deschenes
Plasma Membrane Localization of Ras Requires Class C Vps Proteins and Functional Mitochondria in Saccharomyces cerevisiae
Mol. Cell. Biol., April 15, 2006; 26(8): 3243 - 3255.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Subramanian, C. A. Woolford, E. Drill, M. Lu, and E. W. Jones
Pbn1p: An essential endoplasmic reticulum membrane protein required for protein processing in the endoplasmic reticulum of budding yeast
PNAS, January 24, 2006; 103(4): 939 - 944.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
B. Lo, L. Li, P. Gissen, H. Christensen, P. J. McKiernan, C. Ye, M. Abdelhaleem, J. A. Hayes, M. D. Williams, D. Chitayat, et al.
Requirement of VPS33B, a member of the Sec1/Munc18 protein family, in megakaryocyte and platelet {alpha}-granule biogenesis
Blood, December 15, 2005; 106(13): 4159 - 4166.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Y. Li, D. Gallwitz, and R. Peng
Structure-based Functional Analysis Reveals a Role for the SM Protein Sly1p in Retrograde Transport to the Endoplasmic Reticulum
Mol. Biol. Cell, September 1, 2005; 16(9): 3951 - 3962.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Pulipparacharuvil, M. A. Akbar, S. Ray, E. A. Sevrioukov, A. S. Haberman, J. Rohrer, and H. Kramer
Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules
J. Cell Sci., August 15, 2005; 118(16): 3663 - 3673.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K. C. Sadler, A. Amsterdam, C. Soroka, J. Boyer, and N. Hopkins
A genetic screen in zebrafish identifies the mutants vps18, nf2 and foie gras as models of liver disease
Development, August 1, 2005; 132(15): 3561 - 3572.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Bugnicourt, M. Froissard, K. Sereti, H. D. Ulrich, R. Haguenauer-Tsapis, and J.-M. Galan
Antagonistic Roles of ESCRT and Vps Class C/HOPS Complexes in the Recycling of Yeast Membrane Proteins
Mol. Biol. Cell, September 1, 2004; 15(9): 4203 - 4214.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2004 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.