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MBC in Press, published online ahead of print June 23, 2004
Mol. Biol. Cell 10.1091/mbc.E04-01-0019

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Submitted on January 10, 2004
Revised on May 30, 2004
Accepted on June 10, 2004

Promotion of Neurite and Filopodium Formation by CD47: Roles of Integrins, Rac, and Cdc42

Motoaki Miyashita*{dagger}, Hiroshi Ohnishi*, Hideki Okazawa*, Hiroyasu Tomonaga*, Akiko Hayashi*, Tetsuro-Takahiro Fujimoto{ddagger}, Nobuhiko Furuya{dagger}, and Takashi Matozaki*{sect}

*Biosignal Research Center, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371-8512, Japan; {dagger}Department of Otolaryngology, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan; {ddagger}Department of Hematology and Oncology, Division of Clinical Pharmacotherapeutics, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8551, Japan

Monitoring Editor: Mark Ginsberg

Axon extension during development is guided by many factors, but the signaling mechanisms responsible for its regulation remain largely unknown. We have now investigated the role of the transmembrane protein CD47 in this process in N1E-115 neuroblastoma cells. Forced expression of CD47 induced the formation of neurites and filopodia. Furthermore, an Fc fusion protein containing the extracellular region of the CD47 ligand SHPS-1 induced filopodium formation, and this effect was enhanced by CD47 overexpression. SHPS-1-Fc also promoted neurite and filopodium formation triggered by serum deprivation. Inhibition of Rac or Cdc42 preferentially blocked CD47-induced formation of neurites and filopodia, respectively. Overexpression of CD47 resulted in the activation of both Rac and Cdc42. The extracellular region of CD47 was sufficient for the induction of neurite formation by forced expression, but the entire structure of CD47 was required for enhancement of filopodium formation by SHPS-1-Fc. Neurite formation induced by CD47 was also inhibited by a mAb to the integrin {beta}3 subunit. These results indicate that the interaction of SHPS-1 with CD47 promotes neurite and filopodium formation through the activation of Rac and Cdc42, and that integrins containing the {beta}3 subunit participate in the effect of CD47 on neurite formation.

{sect}Corresponding author. E-mail: matozaki{at}showa.gunma-u.ac.jp






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