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A more recent version of this article appeared on February 1, 2005
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Submitted on February 2, 2004
Accepted on November 9, 2004
Department of Microbiology, University of Marburg and Max-Planck-Institute for Terrestrial Microbiology, D-35043 Marburg, Germany
Monitoring Editor: John Pringle
Polarized growth in filamentous fungi requires the integrity of the microtubule (MT) cytoskeleton. We found that growing MTs in Aspergillus nidulans merge at the center of fast growing tips and discovered that a kinesin motor protein, KipA, related to Tea2p of Schizosaccharomyces pombe, is required for this process. In a 
kipA strain, MT plus ends reach the tip but show continuous lateral movement. Hyphae lose directionality and grow in curves, apparently due to mislocalization of the vesicle supply center (Spitzenkörper) in the apex. GFP-KipA accumulates at MT plus ends, whereas a KipA rigor mutant protein, GFP-KipAG223E, coated MTs evenly. These findings suggest that KipA requires its intrinsic motor activity to reach the MT plus end. Using KipA as a MT plus-end marker, we found bidirectional organization of MTs and determined the locations of MTOCs at nuclei, in the cytoplasm, and at septa.
Institute of Cell & Molecular Biology, University of Edinburgh, Michael Swann Building, The King’s Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland, United Kingdom; Applied Microbiology, University of Karlsruhe, Hertzstrasse 16, D-76187 Karlsruhe, Germany.
Corresponding author.
E-mail: reinhard.fischer{at}bio.uni-karlsruhe.de
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