Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print July 21, 2004
Mol. Biol. Cell 10.1091/mbc.E04-02-0144

A more recent version of this article appeared on October 1, 2004 Originally published as MBC in Press, 10.1091/mbc.E04-02-0144 on July 21, 2004
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E04-02-0144v1
E04-02-0144v2
15/10/4490    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Harcus, D.
Right arrow Articles by Whiteway, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Harcus, D.
Right arrow Articles by Whiteway, M.

Submitted on February 23, 2004
Revised on July 8, 2004
Accepted on July 12, 2004

Transcription Profiling of Cyclic AMP Signaling in Candida albicans

Doreen Harcus*, André Nantel*{dagger}, Anne Marcil*, Tracey Rigby*, and Malcolm Whiteway*{ddagger}{sect}

*Genetics Group, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada H4P 2R2; {dagger}Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 2B2; {ddagger}Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1

Monitoring Editor: Keith Yamamoto

We used transcription profiling in Candida albicans to investigate cellular regulation involving cAMP. We found that many genes require the adenylyl cyclase Cdc35p for proper expression. These include genes encoding ribosomal subunit proteins and RNA polymerase subunit proteins, suggesting that growth could be controlled in part by cAMP mediated modulation of gene expression. Other genes influenced by loss of adenylyl cyclase are involved in metabolism, the cell wall, and stress response, and include a group of genes of unknown function that are unique to C. albicans. The profiles generated by loss of the adenylyl cyclase regulator Ras1p and a downstream effector Efg1p were also examined. The loss of Ras1p function disturbs the expression of a subset of the genes regulated by adenylyl cyclase, suggesting both that the primary role of Ras1p in transcriptional regulation involves its influence on the function of Cdc35p and that there are Ras1p independent roles for Cdc35p. The transcription factor Efg1p is also needed for the expression of many genes; however, these genes are distinct from those modulated by Cdc35p with the exception of a class of hyphal specific genes. Therefore transcription profiling establishes that cAMP plays a key role in the overall regulation of gene expression in C. albicans, and enhances our detailed understanding of the circuitry controlling this regulation.

{sect}Corresponding author. E-mail: malcolm.whiteway{at}cnrc-nrc.gc.ca






Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2004 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.