![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on July 1, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on March 5, 2004
Revised on April 1, 2004
Accepted on April 6, 2004
1 RNA Group/Groupe ARN, Département de Microbiologie et d’Infectiologie, Faculté de Médecine, Université de Sherbrooke, Sherbrooke, Québec, Canada J1H 5N4
* Corresponding author. E-mail address: Sherif.Abou.Elela{at}Usherbrooke.ca.
Members of the double-stranded RNA (dsRNA) specific ribonuclease III (RNase III) family were shown to affect cell division and chromosome segregation, presumably through an RNA interference (RNAi) dependent mechanism. Here we show that in S. cerevisiae, where the RNAi machinery is not conserved, an orthologue of RNase III (Rnt1p) is required for progression of the cell cycle and nuclear division. The deletion of Rnt1p delayed cells in both G1 and G2/M phases of the cell cycle. Nuclear division and positioning at the bud neck were also impaired in 
rnt1 cells. The cell cycle defects were restored by the expression of catalytically inactive Rnt1p, indicating that RNA cleavage is not essential for cell cycle progression. Rnt1p was found to exit from the nucleolus to the nucleoplasm in the G2/M phase and perturbation of its localization pattern delayed the progression of cell division. A single mutation in the Rnt1p N-terminal domain, prevented its accumulation in the nucleoplasm and slowed exit from mitosis without any detectable effects on RNA processing. Together, the data reveal a new role for a class II RNase III in the cell cycle, and suggest that at least some members of the RNase III family possess catalysis independent functions.
This article has been cited by other articles:
|
|
 |
|
  P. Comella, F. Pontvianne, S. Lahmy, F. Vignols, N. Barbezier, A. DeBures, E. Jobet, E. Brugidou, M. Echeverria, and J. Saez-Vasquez Characterization of a ribonuclease III-like protein required for cleavage of the pre-rRNA in the 3'ETS in Arabidopsis Nucleic Acids Res., March 27, 2008; 36(4): 1163 - 1175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Catala, M. Tremblay, E. Samson, A. Conconi, and S. Abou Elela Deletion of Rnt1p Alters the Proportion of Open versus Closed rRNA Gene Repeats in Yeast Mol. Cell. Biol., January 15, 2008; 28(2): 619 - 629. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Larose, N. Laterreur, G. Ghazal, J. Gagnon, R. J. Wellinger, and S. A. Elela RNase III-dependent Regulation of Yeast Telomerase J. Biol. Chem., February 16, 2007; 282(7): 4373 - 4381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ghazal, D. Ge, J. Gervais-Bird, J. Gagnon, and S. Abou Elela Genome-Wide Prediction and Analysis of Yeast RNase III-Dependent snoRNA Processing Signals Mol. Cell. Biol., April 15, 2005; 25(8): 2981 - 2994. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. HENRAS, E. BERTRAND, and G. CHANFREAU A cotranscriptional model for 3'-end processing of the Saccharomyces cerevisiae pre-ribosomal RNA precursor RNA, October 20, 2004; 10(10): 1572 - 1585. [Abstract] [Full Text] [PDF]
|
|
