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A more recent version of this article appeared on July 1, 2004
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Submitted on March 26, 2004
Revised on April 29, 2004
Accepted on April 29, 2004
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||, , , *
Division of Yeast Genetics, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK; Department of Biology, University College London, Gower Street, London, WC1E 6B, UK
Monitoring Editor: Mark Solomon
In animal and yeast cells the mitotic spindle is aligned perpendicularly to the axis of cell division. This ensures that sister chromatids are separated to opposite sides of the cytokinetic actomyosin ring. In fission yeast, spindle rotation is dependent upon the interaction of astral microtubules with the cortical actin cytoskeleton. In this paper we show that addition of Latrunculin A, which prevents spindle rotation, delays the separation of sister chromatids and APC-mediated destruction of spindle associated Securin and Cyclin B. Moreover we find that whereas sister kinetochore pairs normally congress to the spindle midzone before anaphase onset, this congression is disrupted when astral microtubule contact with the actin cytoskeleton is disturbed. By analyzing the timing of kinetochore separation, we find that this anaphase delay requires the Bub3, Mad3 and Bub1 but not the Mad1 or Mad2 spindle assembly checkpoint proteins. In agreement with this we find that Bub1 remains associated with kinetochores when spindles are mis-positioned. These data indicate that, in fission yeast, astral microtubule contact with the medial cell cortex is monitored by a subset of spindle assembly checkpoint proteins. We propose that this checkpoint ensures spindles are properly oriented before anaphase takes place.
Present address: LBCMCP-CNRS UMR5088, Institut d'Exploration Fonctionelle des Génomes (IFR109), Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse, France
||These authors contributed equally to this work
*Corresponding author. E-mail address: jmillar{at}nimr.mrc.ac.uk
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