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MBC in Press, published online ahead of print September 15, 2004
Mol. Biol. Cell 10.1091/mbc.E04-04-0296

A more recent version of this article appeared on November 1, 2004
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Submitted on April 8, 2004
Revised on September 1, 2004
Accepted on September 2, 2004

Stable and Dynamic Axes of Polarity Utilize Distinct Formin Isoforms in Budding Yeast

David Pruyne,* Lina Gao,* Erfei Bi,{dagger} and Anthony Bretscher*{ddagger}

*Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853-2703; {dagger}Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6018

Monitoring Editor: Tim Stearns

Bud growth in yeast is guided by myosin-driven delivery of secretory vesicles from the mother cell to the bud. We find transport occurs along two sets of actin cables assembled by two formin isoforms. The Bnr1p formin assembles cables that radiate from the bud neck into the mother, providing a stable mother-bud axis. These cables also depend on septins at the neck, and are required for efficient transport from the mother to the bud. The Bni1p formin assembles cables that line the bud cortex and target vesicles to varying locations in the bud. Loss of these cables results in morphological defects as vesicles accumulate at the neck. Assembly of these cables depends on continued polarized secretion, suggesting vesicular transport provides a positive feedback signal for Bni1p activation, possibly by rho-proteins. By coupling different formin isoforms to unique cortical landmarks, yeast utilizes common cytoskeletal elements to maintain stable and dynamic axes in the same cell.

{ddagger}Corresponding author. E-mail: apb5{at}cornell.edu






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