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MBC in Press, published online ahead of print July 14, 2004
Mol. Biol. Cell 10.1091/mbc.E04-04-0316

A more recent version of this article appeared on September 1, 2004
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Submitted on April 15, 2004
Revised on June 25, 2004
Accepted on July 1, 2004

The ArfGAP Glo3 Is Required for the Generation of COPI Vesicles

Stephen M. Lewis*, Pak Phi Poon*{dagger}, Richard A. Singer{dagger}, Gerald C. Johnston*, and Anne Spang{ddagger}{sect}

*Department of Microbiology & Immunology and {dagger}Department of Biochemistry & Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 1X5; and {ddagger}Friedrich Miescher Laboratorium, Max-Planck-Gesellschaft, D-72076 Tübingen, Germany

Monitoring Editor: Howard Riezman

The small GTPase Arf and coatomer (COPI) are required for the generation of retrograde transport vesicles. Arf activity is regulated by guanine exchange factors (ArfGEFs) and GTPase-activating proteins (ArfGAPs). The ArfGAPs Gcs1 and Glo3 provide essential overlapping function for retrograde vesicular transport from the Golgi to the ER. We have identified Glo3 as a component of COPI vesicles. Furthermore, we find that a mutant version of the Glo3 protein exerts a negative effect on retrograde transport, even in the presence of the ArfGAP Gcs1. Finally, we present evidence supporting a role for ArfGAP protein in the generation of COPI retrograde transport vesicles.

{sect}Corresponding author. E-mail: anne.spang{at}tuebingen.mpg.de






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