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MBC in Press, published online ahead of print July 28, 2004
Mol. Biol. Cell 10.1091/mbc.E04-04-0330

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Submitted on April 21, 2004
Revised on July 14, 2004
Accepted on July 16, 2004

Protein-Protein Interactions Governing Septin Heteropentamer Assembly and Septin Filament Organization in Saccharomyces cerevisiae

Matthias Versele*, Björn Gullbrand, Mark J. Shulewitz{dagger}, Victor J. Cid{ddagger}, Shirin Bahmanyar{sect}, Raymond E. Chen, Patrick Barth, Tom Alber, and Jeremy Thorner||

Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3202

Monitoring Editor: Anthony Bretscher

Mitotic yeast (S. cerevisiae) cells express five related septins (Cdc3, Cdc10, Cdc11, Cdc12 and Shs1) that form a cortical filamentous collar at the mother-bud neck necessary for normal morphogenesis and cytokinesis. All five possess an N-terminal GTPase domain and, except for Cdc10, a C-terminal extension (CTE) containing a predicted coiled-coil. Here we show that the CTEs of Cdc3 and Cdc12 are essential for their association and for the function of both septins in vivo. Cdc10 interacts with a Cdc3-Cdc12 complex independently of the CTE of either protein. In contrast to Cdc3 and Cdc12, the Cdc11 CTE, which recruits the nonessential septin Shs1, is dispensable for its function in vivo. In addition, Cdc11 forms a stoichiometric complex with Cdc12, independent of its CTE. Reconstitution of various multi-septin complexes and EM analysis reveal that Cdc3, Cdc11 and Cdc12 are all necessary and sufficient for septin filament formation, and presence of Cdc10 causes filament pairing. These data provide novel insights about the connectivity among the five individual septins in functional septin heteropentamers and the organization of septin filaments.

Present addresses: *Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, Katholieke Universiteit Leuven, Leuven-Heverlee B-3001, Belgium; {dagger}Genentech, Inc., South San Francisco, CA 94080; {ddagger}Department of Microbiology, School of Pharmacy, University of Madrid Complutense, Madrid 28040, Spain; {sect}Department of Biological Sciences, Stanford University, Stanford, CA 94305

||Corresponding author. E-mail: jeremy{at}socrates.berkeley.edu






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