![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on January 1, 2005
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on May 6, 2004
Revised on October 22, 2004
Accepted on October 27, 2004
*Department of Biology and Center for Cell Signaling Research, Ewha Women’s University, Seoul 120-750, Korea; Department of Biochemistry, Chonbuk National University Medical School, Chonju 561-182, Korea
Monitoring Editor: Carl-Henrik Heldin
PI-3 kinase and its downstream signaling molecules, PDK-1 and Akt were analyzed in SK-N-SH and SK-N-BE(2) human neuroblastoma cell lines. When cells were stimulated with insulin PI-3 kinase was activated in both cell lines, while the translocation of PDK-1 to the membrane fraction and phosphorylated Akt were observed only in SK-N-SH cells. Analyses of the insulin-mediated reactive oxygen species (ROS) generation and PTEN oxidation indicate that PTEN oxidation occurred in SK-N-SH cells, which can produce ROS, but not in SK-N-BE(2) cells, which cannot increase ROS in response to insulin stimulation. When SK-N-SH cells were pretreated with the NADPH oxidase inhibitor DPI before insulin stimulation, insulin-mediated translocation of PDK-1 to the membrane fraction and phosphorylation of Akt were remarkably reduced while PI-3 kinase activity was not changed significantly. These results indicate that not only PI-3 kinase activation but also inhibition of PTEN by ROS is needed to increase cellular level of PtdIns(3,4,5)P3 for recruiting downstream signaling molecules such as PDK-1 and Akt in insulin-mediated signaling. Moreover, the ROS generated by insulin stimulation mainly contributes to the inactivation of PTEN and not to the activation of PI-3 kinase in the PI-3 kinase/Akt pathway.
Corresponding author.
E-mail: hurkc{at}ewha.ac.kr
This article has been cited by other articles:
|
|
 |
|
  S. S. Barbieri, L. Ruggiero, E. Tremoli, and B. B. Weksler Suppressing PTEN Activity by Tobacco Smoke Plus Interleukin-1{beta} Modulates Dissociation of VE-Cadherin/{beta}-Catenin Complexes in Endothelium Arterioscler. Thromb. Vasc. Biol., April 1, 2008; 28(4): 732 - 738. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Cai, H. Zhong, M. Bosch-Marce, K. Fox-Talbot, L. Wang, C. Wei, M. A. Trush, and G. L. Semenza Complete loss of ischaemic preconditioning-induced cardioprotection in mice with partial deficiency of HIF-1{alpha} Cardiovasc Res, February 1, 2008; 77(3): 463 - 470. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ytrehus Hypoxia-inducible factor 1{alpha}: a new piece in the preconditioning puzzle Cardiovasc Res, February 1, 2008; 77(3): 443 - 444. [Full Text] [PDF]
|
|
|
|
 |
|
 T. Tamguney and D. Stokoe New insights into PTEN J. Cell Sci., December 1, 2007; 120(23): 4071 - 4079. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-C. Lauzier, E. L. Page, M. D. Michaud, and D. E. Richard Differential Regulation of Hypoxia-Inducible Factor-1 through Receptor Tyrosine Kinase Transactivation in Vascular Smooth Muscle Cells Endocrinology, August 1, 2007; 148(8): 4023 - 4031. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Deliri and C. A. McNamara Nox 4 Regulation of Vascular Smooth Muscle Cell Differentiation Marker Gene Expression Arterioscler. Thromb. Vasc. Biol., January 1, 2007; 27(1): 12 - 14. [Full Text] [PDF]
|
|
|
|
 |
|
 D. F. Barber, M. Alvarado-Kristensson, A. Gonzalez-Garcia, R. Pulido, and A. C. Carrera PTEN Regulation, a Novel Function for the p85 Subunit of Phosphoinositide 3-Kinase Sci. Signal., November 21, 2006; 2006(362): pe49 - pe49. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q.-s. Zhu, L. Xia, G. B. Mills, C. A. Lowell, I. P. Touw, and S. J. Corey G-CSF induced reactive oxygen species involves Lyn-PI3-kinase-Akt and contributes to myeloid cell growth Blood, March 1, 2006; 107(5): 1847 - 1856. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Liu and B. L. Fanburg Serotonin-Induced Growth of Pulmonary Artery Smooth Muscle Requires Activation of Phosphatidylinositol 3-Kinase/Serine-Threonine Protein Kinase B/Mammalian Target of Rapamycin/p70 Ribosomal S6 Kinase 1 Am. J. Respir. Cell Mol. Biol., February 1, 2006; 34(2): 182 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Cai and G. L. Semenza PTEN Activity Is Modulated During Ischemia and Reperfusion: Involvement in the Induction and Decay of Preconditioning Circ. Res., December 9, 2005; 97(12): 1351 - 1359. [Abstract] [Full Text] [PDF]
|
|
