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MBC in Press, published online ahead of print July 21, 2004
Mol. Biol. Cell 10.1091/mbc.E04-05-0377

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Submitted on May 8, 2004
Revised on July 9, 2004
Accepted on July 12, 2004

A Dynein Light Intermediate Chain, D1bLIC, Is Required for Retrograde Intraflagellar Transport

Yuqing Hou*, Gregory J. Pazour{dagger}, and George B. Witman*{ddagger}

*Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655; {dagger}Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605

Monitoring Editor: Paul Matsudaira

Intraflagellar transport (IFT), the bidirectional movement of particles along flagella, is essential for flagellar assembly. The motor for retrograde IFT in Chlamydomonas is cytoplasmic dynein 1b, which contains the dynein heavy chain DHC1b and the light IC D1bLIC. To investigate a possible role for the light IC (LIC) in IFT, we identified a d1blic mutant. DHC1b is reduced in the mutant, indicating that D1bLIC is important for stabilizing dynein 1b. The mutant has variable length flagella that accumulate IFT-particle proteins, indicative of a defect in retrograde IFT. Interestingly, the remaining DHC1b is normally distributed in the mutant flagella, strongly suggesting that the defect is in binding of cargo to the retrograde motor rather than in motor activity per se. Cell growth and Golgi apparatus localization and morphology are normal in the mutant, indicating that D1bLIC is involved mainly in retrograde IFT. Like mammalian LICs, D1bLIC has a P-loop at its N-terminus. To investigate the function of this conserved domain, d1blic mutant cells were transformed with constructs designed to express D1bLIC proteins with mutated P-loops. The constructs rescued the mutant cells to a wild-type phenotype, indicating that the function of D1bLIC in IFT is independent of its P-loop.

{ddagger}Corresponding author. E-mail: george.witman{at}umassmed.edu






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