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MBC in Press, published online ahead of print September 8, 2004
Mol. Biol. Cell 10.1091/mbc.E04-05-0411

A more recent version of this article appeared on November 1, 2004
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Submitted on May 18, 2004
Revised on August 26, 2004
Accepted on August 27, 2004

Arf1p Provides an Unexpected Link between COPI Vesicles and mRNA in Saccharomyces cerevisiae

Mark Trautwein,* Jörn Dengjel,{dagger} Markus Schirle,{dagger} and Anne Spang*{ddagger}

*Friedrich Miescher Laboratorium, Max Planck Gesellschaft, D-72076 Tübingen, Germany; {dagger}Universität Tübingen, Interfakultäres Institut, D-72076 Tübingen, Germany

Monitoring Editor: Howard Riezman

The small GTPase Arf1p is involved in different cellular processes that require its accumulation at specific cellular locations. The recruitment of Arf1p to distinct points of action might be achieved by association of Arf1p with different proteins. To identify new interactors of Arf1p, we performed an affinity chromatography with GTP- or GDP-bound Arf1p proteins. A new interactor of Arf1p-GTP was identified as Pab1p, which binds to the polyA-tail of mRNAs. Pab1p was found to associate with purified COPI coated vesicles generated from Golgi membranes in vitro. The stability of the Pab1p-Arf1p complex depends on the presence of mRNA. Both symmetrically distributed mRNAs as well as the asymmetrically localized ASH1 mRNA are found in association with Arf1p. Remarkably, Arf1p and Pab1p are both required to restrict ASH1 mRNA to the bud tip. Arf1p and coatomer play an unexpected role in localizing mRNA independent and downstream of the SHE machinery. Hereby acts the SHE machinery in long-range mRNA transport while COPI vesicles could act as short-range and localization vehicles. The ER-Golgi shuttle might be involved in concentrating mRNA at the ER.

{ddagger}Corresponding author. E-mail: anne.spang{at}tuebingen.mpg.de






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