Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
 QUICK SEARCH:   [advanced]


     


MBC in Press, published online ahead of print October 27, 2004
Mol. Biol. Cell 10.1091/mbc.E04-06-0447

A more recent version of this article appeared on January 1, 2005
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E04-06-0447v1
16/1/292    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Scrittori, L.
Right arrow Articles by Margolis, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Scrittori, L.
Right arrow Articles by Margolis, R.

Submitted on June 4, 2004
Revised on September 22, 2004
Accepted on October 13, 2004

A Small C-Terminal Sequence of Aurora B Is Responsible for Localization and Function

Laetitia Scrittori,*{dagger} Dimitrios Skoufias,{dagger}{ddagger} Fabienne Hans,*{dagger} Véronique Gerson,* Paolo Sassone-Corsi,{sect} Stefan Dimitrov,*|| and Robert Margolis{ddagger}||

*Institut Albert Bonniot, Institut National de la Santé et de la Recherche Médicale, 38706 La Tronche cedex, France; {ddagger}Institut de Biologie Structurale Jean Pierre Ebel (Commissariat à l’Energie Atomique/Centre National de la Recherche Scientifique), 38027 Grenoble cedex 1, France; {sect}Institut de Génétique et de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique - Institut National de la Santé et de la Recherche Médicale -Université Louis Pasteur, 67404 Illkirch-Strasbourg, France

Monitoring Editor: J. Richard McIntosh

Aurora B, a protein kinase required in mitosis, localizes to inner centromeres at metaphase and the spindle midzone in anaphase and is required for proper chromosome segregation and cytokinesis. Aurora A, a paralogue of Aurora B, localizes instead to centrosomes and spindle microtubules. Except for distinct N-termini, Aurora B and Aurora A have highly similar sequences. We have combined siRNA ablation of Aurora B with overexpression of truncation mutants to investigate the role of Aurora B sequence in its function. Reintroduction of Aurora B during siRNA treatment restored its localization and function. This permitted a restoration of function test to determine the sequence requirements for Aurora B targeting and function. Using this rescue protocol, neither N-terminal truncation of Aurora B unique sequence nor substitution with Aurora A N-terminal sequence affected Aurora B localization or function. Truncation of unique Aurora B C-terminal sequence from terminal residue 344 to residue 333 was without effect, but truncation to 326 abolished localization and function. Deletion of residues 326-333 completely abolished localization and blocked cells at prometaphase, establishing this sequence as critical to Aurora B function. Our findings thus establish a small sequence as essential for the distinct localization and function of Aurora B.


{dagger}These authors contributed equally to this work.

||Corresponding authors. E-mail: stefan.dimitrov{at}ujf-grenoble.fr E-mail: Margolis{at}ibs.fr







Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2004 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.