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MBC in Press, published online ahead of print September 15, 2004
Mol. Biol. Cell 10.1091/mbc.E04-06-0522

A more recent version of this article appeared on December 1, 2004
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Submitted on June 25, 2004
Accepted on September 1, 2004

Apatite-mediated Actin Dynamics in Resorbing Osteoclasts

Frédéric Saltel,*{dagger} Olivier Destaing,*{dagger} Frédéric Bard,{ddagger} Diane Eichert,{sect} and Pierre Jurdic*||

*Laboratoire de Biologie Moléculaire de la Cellule, ENS/CNRS 5161, IFR 128 Biosciences Lyon-Gerland, 69007 Lyon, France; {ddagger}Cell and Developmental Biology Department, University of California, San Diego, CA 92093-0347; {sect}European Synchrotron Radiation Facility, BP 220 38043 Grenoble, France

Monitoring Editor: Thomas Pollard

The actin cytoskeleton is essential for osteoclast main function, bone resorption. Two different organizations of actin have been described in osteoclasts, the podosomes belt corresponding to numerous F-actin columns arranged at the cell periphery, and the sealing zone, defined as a unique large band of actin. To compare the role of these two different actin organizations, we imaged osteoclasts on various substrata: glass, dentin and apatite. Using primary osteoclasts expressing GFP-actin we found that podosome belts and sealing zones, both very dynamic actin structures, were present in mature osteoclasts; podosome belts were observed only in spread osteoclasts adhering onto glass, whereas sealing zone were seen in apico-basal polarized osteoclasts adherent on mineralized matrix. Dynamic observations of several resorption cycles of osteoclasts seeded on apatite revealed that podosomes do not fuse together to form the sealing zone; osteoclasts alternate successive stationary polarized resorption phases with a sealing zone and migration, nonresorption phases without any specific actin structure; and apatite itself promotes sealing zone formation though c-src and Rho signaling. Finally, our work suggests that apatite-mediated sealing zone formation is dependent of both c-src and Rho whereas apico-basal polarization requires only Rho.

{dagger}These authors contributed equally to this work.

||Corresponding author. E-mail: pjurdic{at}ens-lyon.fr






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