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MBC in Press, published online ahead of print September 15, 2004
Mol. Biol. Cell 10.1091/mbc.E04-07-0571

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Submitted on July 8, 2004
Revised on August 26, 2004
Accepted on August 31, 2004

A NIMA-related kinase, Fa2p, Localizes to a Novel Site in the Proximal Cilia of Chlamydomonas and Mouse Kidney Cells

Moe R. Mahjoub, M. Qasim Rasi, and Lynne M. Quarmby*

Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S6

Monitoring Editor: Trisha Davis

Polycystic kidney disease and related syndromes involve dysregulation of cell proliferation in conjunction with ciliary defects. The relationship between cilia and cell cycle is enigmatic, but may involve regulation by the NIMA-family of kinases (Neks). We previously showed that a Nek, Fa2p, is important for ciliary function and cell cycle in Chlamydomonas. We now show that Fa2p localizes to an important regulatory site at the proximal end of cilia in both Chlamydomonas and a mouse kidney cell line. Fa2p is also associated with the proximal end of centrioles. Its localization is dynamic during the cell cycle, following a similar pattern in both cell types. The cell cycle function of Fa2p is kinase-independent, whereas its ciliary function is kinase-dependent. Mice with mutations in Nek1 or Nek8 have cystic kidneys; therefore, our discovery that a member of this phylogenetic group of Nek proteins is localized to the same sites in Chlamydomonas and kidney epithelial cells suggests that Neks play conserved roles in the coordination of cilia and cell cycle progression.

*Corresponding author. E-mail: quarmby{at}sfu.ca






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