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MBC in Press, published online ahead of print February 25, 2005
Mol. Biol. Cell 10.1091/mbc.E04-07-0583

A more recent version of this article appeared on April 1, 2005 Originally published as MBC in Press, 10.1091/mbc.E04-07-0583 on January 19, 2005
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Submitted on July 13, 2004
Accepted on January 11, 2005

The Functionally Conserved Nucleoporins Nup124p from Fission Yeast and the Human Nup153 Mediate Nuclear Import and Activity of the Tf1 Retrotransposon and HIV-1 Vpr

Padmapriya Varadarajan,*{dagger} Sundarasamy Mahalingam,*{dagger}{ddagger} Peiyun Liu,*{dagger} Sarah Boon Hsi Ng,* Sheetal Gandotra,* Desmond Suresh Kumar Dorairajoo,* and David Balasundaram*{dagger}{sect}

*Laboratory of Nucleopore Biology, Institute of Molecular and Cell Biology, Singapore 138673, Republic of Singapore; {ddagger}Laboratory of Molecular Virology, Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500076, India

Monitoring Editor: Sandra Schmid

We report that the fission yeast nucleoporin Nup124p is required for the nuclear import of both, retrotransposon Tf1-Gag as well as the retroviral HIV-1 Vpr. Failure to import Tf1-Gag into the nucleus in a nup124 null mutant resulted in complete loss of Tf1 transposition. Similarly, nuclear import of HIV-1 Vpr was impaired in nup124 null mutant strains and cells became resistant to Vpr’s cell-killing activity. On the basis of protein domain similarity, the human nucleoporin Nup153 was identified as a putative homolog of Nup124p. We demonstrate that in vitro translated Nup124p and Nup153 coimmunoprecipitate Tf1-Gag or HIV-1Vpr. Though full-length Nup153 was unable to complement the Tf1 transposition defect in a nup124 null mutant, we provide evidence that both nucleoporins share a unique N-terminal domain, Nup124pAA264-454 and Nup153AA448-634 that is absolutely essential for Tf1 transposition. Epigenetic overexpression of this domain in a wild-type (nup124+) background blocked Tf1 activity implying that sequences from Nup124p and the human Nup153 challenged the same pathway affecting Tf1 transposition. Our results establish a unique relationship between two analogous nucleoporins Nup124p and Nup153 wherein the function of a common domain in retrotransposition is conserved.

{dagger}These authors contributed equally to this work.

{sect}Corresponding author. David Balasundaram (davidb{at}imcb.a-star.edu.sg)






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