Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print March 16, 2005
Mol. Biol. Cell 10.1091/mbc.E04-07-0596

A more recent version of this article appeared on June 1, 2005
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E04-07-0596v1
16/6/2704    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Puri, C.
Right arrow Articles by Tacchetti, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Puri, C.
Right arrow Articles by Tacchetti, C.

Submitted on July 16, 2004
Revised on March 7, 2005
Accepted on March 8, 2005

Relationships between EGFR Signaling-competent and Endocytosis-competent Membrane Microdomains

Claudia Puri,*{dagger}{ddagger} Daniela Tosoni,{dagger}{ddagger}{sect} Riccardo Comai,*{dagger} Andrea Rabellino,*{dagger} Daniela Segat,* Federico Caneva,* Paola Luzzi,*{dagger} Pier Paolo Di Fiore,{dagger}{sect}|| and Carlo Tacchetti*{dagger}

*MicroScoBio Research Center, Department of Experimental Medicine, University of Genoa, 16132 Genoa, Italy; {dagger}IFOM, FIRC Institute of Molecular Oncology, 20139 Milan, Italy; {sect}Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy; ||Department of Medicine, Surgery and Dentistry, University of Milan, 20142 Milan, Italy

Monitoring Editor: Juan S. Bonifacino

Membrane microdomains, the so-called lipid rafts, function as platforms to concentrate receptors and assemble the signal transduction machinery. Internalization, in most cases, is carried out by different specialized structures, the clathrin-coated pits. Here, we show that several endocytic proteins are efficiently recruited to morphologically identified plasma membrane lipid rafts, upon activation of the epidermal growth factor (EGF) receptor (EGFR), a receptor tyrosine kinase. Analysis of detergent-resistant membrane fractions revealed that the EGF-dependent association of endocytic proteins with rafts is as efficient as that of signaling effector molecules, such as Grb2 or Shc. Finally, the EGFR, but not the nonsignaling transferrin receptor, could be localized in nascent coated pits that almost invariably contained raft membranes. Thus, specialized membrane microdomains have the ability to assemble both the molecular machineries necessary for intracellular propagation of EGFR effector signals and for receptor internalization.

{ddagger}These authors contributed equally to this study.

Address correspondence to: Carlo Tacchetti (carlo.tacchetti{at}unige.it)






Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.