Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print February 25, 2005
Mol. Biol. Cell 10.1091/mbc.E04-08-0744

A more recent version of this article appeared on April 1, 2005 Originally published as MBC in Press, 10.1091/mbc.E04-08-0744 on February 9, 2005
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E04-08-0744v1
E04-08-0744v2
16/4/1859    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fournier, H.-N.
Right arrow Articles by Albiges-Rizo, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fournier, H.-N.
Right arrow Articles by Albiges-Rizo, C.

Submitted on August 27, 2004
Accepted on January 26, 2005

Nuclear Translocation of ICAP-1 Stimulates Cellular Proliferation

Henri-Noël Fournier,* Sandra Dupé-Manet,* Daniel Bouvard,* Frédéric Luton,{dagger} Simona Degani,{ddagger} Marc R. Block,* Saverio Francesco Retta,{ddagger} and Corinne Albiges-Rizo*

*Laboratoire d’Etude de la Différenciation et de l’Adhérence Cellulaires, UMR UJF/CNRS 5538 Institut Albert Bonniot, Faculté de Médecine de Grenoble, Domaine de la Merci, 38706 La Tronche Cedex, France; {dagger}Dynamique Moléculaire des Compartiments Cellulaires, Institut de Pharmacologie du CNRS, 06560 Sophia Antipolis, France; {ddagger}Department of Genetics, Biology and Biochemistry, University of Torino, 10149 Turin, Italy

Monitoring Editor: Mark Ginsberg

Integrin cytoplasmic domain-associated protein 1 (ICAP-1) has been shown to interact specifically with the beta1 integrin cytoplasmic domain and to control cell spreading on fibronectin. Interestingly, ICAP-1 is also observed in the nucleus, by immunocytochemical staining and after biochemical cell fractionation suggesting that it has additional roles that have yet to be determined. We show that the nucleocytoplasmic shuttling capability of ICAP-1 is dependent on a functional nuclear localization signal. In addition, overexpression of beta1 integrin strongly reduced this nuclear localization, suggesting that integrin activity could modulate ICAP-1 shuttling by sequestering it in the cytoplasm. Indeed, the nuclear localization of ICAP-1 is dependent on the stage of cell spreading on fibronectin and we also show that ICAP-1 expression stimulates cellular proliferation in a fibronectin dependent manner. This function is dependent on its nuclear localization. Moreover, ICAP-1 is able to activate the c-myc promoter in vitro. Taken together, these results demonstrate that ICAP-1 shuttles between the nucleus and cytoplasm in a beta 1 integrin dependent manner. It could act as a messenger that relays information from sites of integrin dependent-cell adhesion to the nucleus for controlling gene expression and cell proliferation.

Address correspondence to: Corinne Albiges-Rizo (corinne.albiges-rizo{at}ujf-grenoble.fr)






Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.