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MBC in Press, published online ahead of print October 20, 2004
Mol. Biol. Cell 10.1091/mbc.E04-09-0791

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Submitted on September 9, 2004
Accepted on October 13, 2004

Proteomic Analysis of the Arabidopsis Nucleolus Suggests Novel Nucleolar Functions

Alison F. Pendle,* Gillian P. Clark,{dagger} Reinier Boon,* Dominika Lewandowska,{dagger} Yun Wah Lam,{ddagger} Jens Andersen,{sect} Matthias Mann,{sect} Angus I. Lamond,{ddagger} John W.S. Brown,{dagger} and Peter J. Shaw*||

*John Innes Centre, Norwich NR4 7UH, United Kingdom; {dagger}Scottish Crop Research Institute, Dundee DD2 5DA, Scotland; {ddagger}University of Dundee, Dundee DD1 5HN, Scotland; {sect}University of Southern Denmark, DK-5230 Odense, Denmark

Monitoring Editor: Joseph Gall

The eukaryotic nucleolus is involved in ribosome biogenesis and a wide range of other RNA metabolism and cellular functions. An important step in the functional analysis of the nucleolus is to determine the complement of proteins of this nuclear compartment. Here, we describe the first proteomic analysis of plant (Arabidopsis thaliana) nucleoli, in which we have identified 217 proteins. This allows a direct comparison of the proteomes of an important nuclear structure between two widely divergent species - human and Arabidopsis. The comparison identified many common proteins, plant-specific proteins, proteins of unknown function found in both proteomes and proteins which were nucleolar in plants but nonnucleolar in human. Seventy two proteins were expressed as GFP fusions and 87% showed nucleolar or nucleolar-associated localization. In a striking and unexpected finding, we have identified six components of the postsplicing exon-junction complex (EJC) involved in mRNA export and nonsense-mediated decay (NMD)/mRNA surveillance. This association was confirmed by GFP-fusion protein localization. These results raise the possibility that in plants, nucleoli may have additional functions in mRNA export or surveillance.

||Corresponding author. E-mail: peter.shaw{at}bbsrc.ac.uk






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