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A more recent version of this article appeared on May 1, 2005
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Submitted on September 23, 2004
Revised on February 4, 2005
Accepted on February 6, 2005
*CRBM, CNRS FRE2593, 34293 Montpellier, France; IPMC, CNRS, Sophia-Antipolis, 06560 Valbonne, France
Monitoring Editor: Martin A. Schwartz
Cadherins are homophilic cell-cell adhesion molecules implicated in cell growth, differentiation and organization into tissues during embryonic development. They accumulate at cell-cell contact sites and act as adhesion-activated signaling receptors. Here we show that the dynamic assembly of N-cadherin at cell-cell contacts involves lipid rafts. In C2C12 myoblasts, immunofluorescence and biochemical experiments demonstrate that N-cadherin present at cell-cell contacts is colocalized with lipid rafts. Disruption of lipid rafts leads to the inhibition of cell-cell adhesion and disorganization of N-cadherin-dependent cell-cell contacts without modifying the association of N-cadherin with catenins and its availability at the plasma membrane. Fluorescent Recovery After Photobleaching (FRAP) experiments demonstrate that at the dorsal plasma membrane, lipid rafts are not directly involved in the diffusional mobility of N-cadherin. In contrast, at cell-cell junctions N-cadherin association with lipid rafts allows its stabilization enabling the formation of a functional adhesive complex. We show that lipid rafts, as homophilic interaction and F-actin association, stabilize cadherin-dependent adhesive complexes. Homophilic interactions and F-actin association of N-cadherin are both required for its association to lipid rafts. We thus identify lipid rafts as new regulators of cadherin-mediated cell adhesion.
These two authors contributed equally to this work.
Present address: IPBS, UMR 5089, 205 Route de Narbonne, 31077 Toulouse, France.
Address correspondence to:
Cécile Gauthier-Rouvière (cecile.gauthier{at}crbm.cnrs.fr)
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