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MBC in Press, published online ahead of print January 12, 2005
Mol. Biol. Cell 10.1091/mbc.E04-10-0885

A more recent version of this article appeared on March 1, 2005
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Submitted on October 8, 2004
Revised on December 10, 2004
Accepted on December 18, 2004

Distinct LIN-10 Domains Are Required for Its Neuronal Function, Its Epithelial Function, and Its Synaptic Localization

Doreen R. Glodowski,* Tricia Wright,* Keri Martinowich,* Howard Chia-Hao Chang, Douglas Beach, and Christopher Rongo{dagger}

The Waksman Institute, Department of Genetics, Rutgers University, Piscataway, NJ 08854

Monitoring Editor: Guido Guidotti

AMPA-type glutamate receptors (AMPARs) mediate excitatory neurotransmission at neuronal synapses, and their regulated localization plays a role in synaptic plasticity. In C. elegans, the PDZ and PTB domain-containing protein LIN-10 is required both for the synaptic localization of the AMPAR subunit GLR-1 and for vulval fate induction in epithelia. Here we examine the role that different LIN-10 domains play in GLR-1 localization. We find that an amino-terminal region of LIN-10 directs LIN-10 protein localization to the Golgi and to synaptic clusters. In addition, mutations in the carboxy-terminal PDZ domains prevent LIN-10 from regulating GLR-1 localization in neurons, but do not prevent LIN-10 from functioning in the vulval epithelia. A mutation in the amino-terminus prevents the protein from functioning in the vulval epithelia, but does not prevent it from functioning to regulate GLR-1 localization in neurons. Finally, we show that human Mint2 can substitute for LIN-10 to facilitate GLR-1 localization in neurons, and that the Mint2 amino-terminus is critical for this function. Taken together, our data suggest that LIN-10 uses distinct modular domains for its functions in neurons and epithelial cells, and that during evolution its vertebrate ortholog, Mint2, has retained the ability to direct AMPAR localization in neurons.

*These authors contributed equally to this work.

{dagger}Corresponding author. E-mail: rongo{at}waksman.rutgers.edu






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