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MBC in Press, published online ahead of print February 2, 2005
Mol. Biol. Cell 10.1091/mbc.E04-10-0934

A more recent version of this article appeared on April 1, 2005 Originally published as MBC in Press, 10.1091/mbc.E04-10-0934 on February 25, 2005
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Submitted on October 27, 2004
Accepted on January 21, 2005

Meiotic S-Phase Damage Activates Recombination without Checkpoint Arrest

Daniel G. Pankratz*{dagger}{ddagger} and Susan L. Forsburg*{sect}

*Molecular & Cell Biology Laboratory, The Salk Institute, La Jolla, CA 92037; {dagger}Division of Biology, University of California, San Diego, La Jolla, CA 92093-0346; {sect}Molecular and Computational Biology Section, University of Southern California, Los Angeles, CA 90089-1340

Monitoring Editor: Mark Solomon

Checkpoints operate during meiosis to ensure the completion of DNA synthesis and programmed recombination before the initiation of meiotic divisions. Studies in the fission yeast Schizosaccharomyces pombe suggest that the meiotic response to DNA damage due to a failed replication checkpoint response differs substantially from the vegetative response, and may be influenced by the presence of homologous chromosomes. The checkpoint responses to DNA damage during fission yeast meiosis are not well characterized. Here we report that DNA damage induced during meiotic S-phase does not activate checkpoint arrest. We also find that in wild-type cells, markers for DNA breaks can persist at least to the first meiotic division. We also observe increased spontaneous S-phase damage in checkpoint mutants, which is repaired by recombination without activating checkpoint arrest. Our results suggest that fission yeast meiosis is exceptionally tolerant of DNA damage, and that some forms of spontaneous S-phase damage can be repaired by recombination without activating checkpoint arrest.

{ddagger}Present address: Oregon Health & Science University, NRC3, 3181 SW Sam Jackson Park Road, Portland, OR 97239.

Address correspondence to: Susan L. Forsburg (forsburg{at}usc.edu)




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