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A more recent version of this article appeared on July 1, 2005
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Submitted on November 16, 2004
Revised on March 15, 2005
Accepted on April 5, 2005
*Programme in Cell Biology, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada; Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada
Monitoring Editor: Ralph Isberg
Coronin-1 is an actin-associated protein whose function in actin dynamics has remained obscure. All coronin proteins have a variable N terminal domain, followed by WD repeats and a C-terminal coiled-coil dimerization domain. Transfection of GFP-coronin-1 into RAW 264.7 cells revealed that coronin rapidly and transiently associates with the phagosome. To determine if coronin is involved in mammalian phagocytosis we used a dominant-negative approach by expressing only the central WD domains. However, this caused cell rounding and dissociation from the substratum, hampering analysis of their phenotype. We therefore developed TAT-fusion constructs of coronin-1 WD domains to acutely introduce the recombinant protein fragment into live cells. We show that while TAT-WD has no effect on binding of opsonized red blood cells to RAW 264.7 cells, receptor clustering or several downstream signaling events, lamellipodial extensions and actin accumulation at the base of the bound particle were diminished. Furthermore, Arp3 accumulation at the phagosome was impaired following TAT-WD treatment. Interestingly, while coronin-1 also accumulates at the sites of actin remodeling associated with Salmonella invasion, TAT-WD had no effect on this process. Together, our data demonstrates that coronin-1 is required for an early step in phagosome formation, consistent with a role in actin polymerization.
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