![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on April 1, 2005 Originally published as MBC in Press, 10.1091/mbc.E04-11-1035 on January 19, 2005
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on November 30, 2004
Revised on January 4, 2005
Accepted on January 7, 2005
Life Sciences Institute and Departments of Molecular, Cellular, and Developmental Biology and Biological Chemistry, University of Michigan, Ann Arbor, MI 48109
Monitoring Editor: Suresh Subramani
Proteins are selectively packaged into vesicles at specific sites and then delivered correctly to the various organelles where they function, which is critical to the proper physiology of each organelle. The precursor form of the vacuolar hydrolase aminopeptidase I (prApe1) is a selective cargo molecule of the cytoplasm to vacuole targeting (Cvt) pathway and autophagy. Precursor Ape1 along with its receptor Atg19 forms the Cvt complex, which is transported to the preautophagosomal structure (PAS), the putative site of Cvt vesicle formation, in a process dependent on Atg11. Here we show that this interaction occurs through the Atg11 C terminus; subsequent recruitment of the Cvt complex to the PAS depends on central regions within Atg11. Atg11 was shown to physically link several proteins, although the timing of those interactions and their importance are unknown. Our mapping shows that the Atg11 coiled-coil domains are involved in its self-assembly, and the interaction with other proteins including two previously unidentified partners, Atg17 and Atg20. Atg11 mutants defective in the transport of the Cvt complex to the PAS affect the localization of other Atg components, supporting the idea that the cargo facilitates the organization of the PAS in selective autophagy. These findings suggest that Atg11 plays an integral role in connecting cargo molecules with components of the vesicle-forming machinery.
This article has been cited by other articles:
|
|
 |
|
  T. Kanki and D. J. Klionsky Mitophagy in Yeast Occurs through a Selective Mechanism J. Biol. Chem., November 21, 2008; 283(47): 32386 - 32393. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Geng, M. Baba, U. Nair, and D. J. Klionsky Quantitative analysis of autophagy-related protein stoichiometry by fluorescence microscopy J. Cell Biol., October 23, 2008; 182(1): 129 - 140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Krick, Y. Muehe, T. Prick, S. Bremer, P. Schlotterhose, E.-L. Eskelinen, J. Millen, D. S. Goldfarb, and M. Thumm Piecemeal Microautophagy of the Nucleus Requires the Core Macroautophagy Genes Mol. Biol. Cell, October 1, 2008; 19(10): 4492 - 4505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Cao, H. Cheong, H. Song, and D. J. Klionsky In vivo reconstitution of autophagy in Saccharomyces cerevisiae J. Cell Biol., August 26, 2008; 182(4): 703 - 713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Cheong, U. Nair, J. Geng, and D. J. Klionsky The Atg1 Kinase Complex Is Involved in the Regulation of Protein Recruitment to Initiate Sequestering Vesicle Formation for Nonspecific Autophagy in Saccharomyces cerevisiae Mol. Biol. Cell, February 1, 2008; 19(2): 668 - 681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-Y. Chang and W.-P. Huang Atg19 Mediates a Dual Interaction Cargo Sorting Mechanism in Selective Autophagy Mol. Biol. Cell, March 1, 2007; 18(3): 919 - 929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. He, H. Song, T. Yorimitsu, I. Monastyrska, W.-L. Yen, J. E. Legakis, and D. J. Klionsky Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast J. Cell Biol., December 18, 2006; 175(6): 925 - 935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yorimitsu, U. Nair, Z. Yang, and D. J. Klionsky Endoplasmic Reticulum Stress Triggers Autophagy J. Biol. Chem., October 6, 2006; 281(40): 30299 - 30304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Nair and D. J. Klionsky Molecular Mechanisms and Regulation of Specific and Nonspecific Autophagy Pathways in Yeast J. Biol. Chem., December 23, 2005; 280(51): 41785 - 41788. [Full Text] [PDF]
|
|
