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A more recent version of this article appeared on April 1, 2005 Originally published as MBC in Press, 10.1091/mbc.E04-12-1052 on February 25, 2005
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Submitted on December 10, 2004
Accepted on January 11, 2005
*Cell Biology Laboratory, Department of Biochemistry, BioSciences Institute, National University of Ireland, Cork, Ireland; Division of Rheumatology and Immunology, Brigham and Women’s Hospital, Boston, MA 02115
Monitoring Editor: Carl-Henrik Heldin
By comparing differential gene expression in the IGF-IR null cell fibroblast cell line (R- cells) with cells over expressing the IGF-IR (R+ cells) we identified the Mystique gene expressed as alternatively spliced variants. The human homologue of Mystique is located on chromosome 8p21.2 and encodes a PDZ LIM domain protein (PDLIM2). GFP-Mystique isoform were colocalized at cytoskeleton focal contacts with 
actinin and 
1-integrin. Only one isoform of endogenous human Mystique protein, Mystique 2 was detected in cell lines. Mystique 2 was more abundant in nontransformed MCF10A breast epithelial cells than in MCF-7 breast carcinoma cells, and was induced by IGF-I and cell adhesion. Over expression of Mystique 2 in MCF-7 cells suppressed colony formation in soft agarose, and enhanced cell adhesion to collagen and fibronectin. Point mutation of either the PDZ or LIM domain was sufficient to reverse suppression of colony formation, but mutation of the PDZ domain alone was sufficient to abolish enhanced adhesion. Knockdown of Mystique 2 with siRNA abrogated both adhesion and migration in MCF10A and MCF-7 cells. The data indicate that Mystique is an IGF-IR-regulated adapter protein located at the actin cytoskeleton that is necessary for the migratory capacity of epithelial cells.
Corresponding author.
E-mail: r.oconnor{at}ucc.ie
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