![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on July 1, 2005
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on January 21, 2005
Revised on April 18, 2005
Accepted on April 20, 2005
*Division of Molecular Medicine, Wadsworth Center, Albany, NY 12201; Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, NY 12201
Monitoring Editor: J. Richard McIntosh
Overexpression of dynein fragments in Dictyostelium induces the movement of the entire interphase microtubule array. Centrosomes in these cells circulate through the cytoplasm at rates between 0.4 and 2.5 µm/s, and are trailed by a comet-tail like arrangement of the microtubule array. Previous work suggested that these cells use a dynein-mediated pulling mechanism to generate this dramatic movement, and that similar forces are at work to maintain the interphase MTOC position in wild-type cells. In the present study, we address the nature of the forces used to produce microtubule movement. We have used a laser microbeam to sever the connection between the motile centrosomes and trailing microtubules, demonstrating that the major force for such motility results from a pushing on the microtubules. We eliminate the possibility that microtubule assembly/disassembly reactions are significant contributors to this motility, and suggest that the cell cortex figures prominently in locating force-producing molecules. Our findings indicate that interphase microtubules in Dictyostelium are subject to both dynein and kinesin-like forces, and that these act in concert to maintain centrosome position in the cell and to support the radial character of the microtubule network.
This article has been cited by other articles:
|
|
 |
|
  L. Tang, J. Franca-Koh, Y. Xiong, M.-Y. Chen, Y. Long, R. M. Bickford, D. A. Knecht, P. A. Iglesias, and P. N. Devreotes tsunami, the Dictyostelium homolog of the Fused kinase, is required for polarization and chemotaxis Genes & Dev., August 15, 2008; 22(16): 2278 - 2290. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Fink and G. Steinberg Dynein-dependent Motility of Microtubules and Nucleation Sites Supports Polarization of the Tubulin Array in the Fungus Ustilago maydis Mol. Biol. Cell, July 1, 2006; 17(7): 3242 - 3253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Myers, Y. He, T. P. Hasaka, and P. W. Baas Microtubule Transport in the Axon: Re-thinking a Potential Role for the Actin Cytoskeleton Neuroscientist, April 1, 2006; 12(2): 107 - 118. [Abstract] [PDF]
|
|
|
|
 |
|
 K. D. Girard, S. C. Kuo, and D. N. Robinson Dictyostelium myosin II mechanochemistry promotes active behavior of the cortex on long time scales PNAS, February 14, 2006; 103(7): 2103 - 2108. [Abstract] [Full Text] [PDF]
|
|
