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MBC in Press, published online ahead of print July 12, 2005
Mol. Biol. Cell 10.1091/mbc.E05-01-0081

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Submitted on January 31, 2005
Revised on June 8, 2005
Accepted on June 29, 2005

PAK5 Kinase Is an Inhibitor of MARK/Par-1, Which Leads to Stable Microtubules and Dynamic Actin

Dorthe Matenia,* Bettina Griesshaber,* Xiao-yu Li, Anja Thiessen, Cindy Johne, Jian Jiao, Eckhard Mandelkow, and Eva-Maria Mandelkow

Unit for Structural Molecular Biology, Max-Planck-Institute, 22607 Hamburg, Germany

Monitoring Editor: J. Richard McIntosh

MARK/Par-1 is a kinase involved in development of embryonic polarity. In neurons, MARK phosphorylates tau protein and causes its detachment from microtubules, the tracks of axonal transport. Because the target sites of MARK on tau occur at an early stage of Alzheimer neurodegeneration we searched for interaction partners of MARK. Here we report that MARK2 is negatively regulated by PAK5, a neuronal member of the p21-activated kinase family. PAK5 suppresses the activity of MARK2 toward its target, tau protein. The inhibition requires the binding between the PAK5 and MARK2 catalytic domains, but does not require phosphorylation. In transfected chinese hamster ovary (CHO) cells both kinases show a vesicular distribution with partial colocalization on endosomes containing AP-1/2. While MARK2 transfected alone destabilizes microtubules and stabilizes actin stress fibers, PAK5 keeps microtubules stable through the down-regulation of MARK2 but destabilizes the F-actin network so that stress fibers and focal adhesions disappear and cells develop filopodia. The results point to an inverse relationship between actin-related and microtubule-related signaling by the PAK5 and MARK2 pathways that affect both cytoskeletal networks.

*These authors contributed equally to this work.

Address correspondence to: Eva-Maria Mandelkow (mand{at}mpasmb.desy.de)




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