Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print June 22, 2005
Mol. Biol. Cell 10.1091/mbc.E05-02-0116

A more recent version of this article appeared on September 1, 2005
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Table 1
Right arrow All Versions of this Article:
E05-02-0116v1
16/9/4225    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Martin, M.
Right arrow Articles by Saxton, W. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Martin, M.
Right arrow Articles by Saxton, W. M.

Submitted on February 10, 2005
Revised on June 13, 2005
Accepted on June 15, 2005

Abl Tyrosine Kinase and Its Substrate Ena/VASP Have Functional Interactions with Kinesin-1

MaryAnn Martin,*{dagger} Shawn M. Ahern-Djamali,{dagger}{ddagger} F. Michael Hoffmann,{ddagger} and William M. Saxton*

*Department of Biology, Indiana University, Bloomington, IN 47405; {ddagger}McArdle Laboratory, University of Wisconsin, Madison, WI 53706

Monitoring Editor: John Pringle

Relatively little is known about how microtubule motors are controlled or about how the functions of different cytoskeletal systems are integrated. A yeast two-hybrid screen for proteins that bind to Drosophila Enabled (Ena), an actin polymerization factor that is negatively regulated by Abl tyrosine kinase, identified kinesin heavy chain (Khc), a member of the kinesin-1 subfamily of microtubule motors. Coimmunoprecipitation from Drosophila cytosol confirmed a physical interaction between Khc and Ena. Kinesin-1 motors can carry organelles and other macromolecular cargoes from neuronal cell bodies toward terminals in fast-axonal-transport. Ena distribution in larval axons was not affected by mutations in the Khc gene, suggesting that Ena is not itself a fast transport cargo of Drosophila kinesin-1. Genetic interaction tests showed that in a background sensitized by reduced Khc gene dosage, a reduction in Abl gene dosage caused distal paralysis and axonal swellings. A concomitant reduction in ena dosage rescued those defects. These results suggest that Ena/VASP, when not inhibited by the Abl pathway, can bind Khc and reduce its transport activity in axons.

{dagger}These authors contributed equally to this work.

Address correspondence to: William M. Saxton (bsaxton{at}bio.indiana.edu)




This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
N. Hirokawa and Y. Noda
Intracellular Transport and Kinesin Superfamily Proteins, KIFs: Structure, Function, and Dynamics
Physiol Rev, July 1, 2008; 88(3): 1089 - 1118.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
V. L. Goss, K. A. Lee, A. Moritz, J. Nardone, E. J. Spek, J. MacNeill, J. Rush, M. J. Comb, and R. D. Polakiewicz
A common phosphotyrosine signature for the Bcr-Abl kinase
Blood, June 15, 2006; 107(12): 4888 - 4897.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
J. G. Gindhart
Towards an understanding of kinesin-1 dependent transport pathways through the study of protein-protein interactions
Brief Funct Genomic Proteomic, March 1, 2006; 5(1): 74 - 86.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P. J. Hollenbeck and W. M. Saxton
The axonal transport of mitochondria
J. Cell Sci., December 1, 2005; 118(23): 5411 - 5419.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.