Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print May 25, 2005
Mol. Biol. Cell 10.1091/mbc.E05-02-0120

A more recent version of this article appeared on August 1, 2005
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Figures
Right arrow All Versions of this Article:
E05-02-0120v1
16/8/3467    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Choudhury, R.
Right arrow Articles by Lowe, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Choudhury, R.
Right arrow Articles by Lowe, M.

Submitted on February 11, 2005
Revised on May 9, 2005
Accepted on May 12, 2005

Lowe Syndrome Protein OCRL1 Interacts with Clathrin and Regulates Protein Trafficking between Endosomes and the Trans-Golgi Network

Rawshan Choudhury,* Aipo Diao,* Fang Zhang,{dagger} Evan Eisenberg,{dagger} Agnes Saint-Pol,{ddagger} Catrin Williams,* Athanasios Konstantakopoulos,{sect} John Lucocq,{sect} Ludger Johannes,{ddagger} Catherine Rabouille,|| Lois E. Greene,{dagger} and Martin Lowe*

*Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom; {dagger}Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892; {ddagger}Traffic and Signaling Laboratory, Department of Cellular Compartmentalization and Dynamics, UMR 144 Curie/CNRS, Curie Institute, F-75248 Paris Cedex 05, France; ||UMC Utrecht, Department of Cell Biology, AZU, 3584 CX Utrecht, The Netherlands; {sect}School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom

Monitoring Editor: Sean Munro

Oculocerebrorenal syndrome of Lowe is caused by mutation of OCRL1, a phosphatidylinositol 4,5-bisphosphate 5-phosphatase localized at the Golgi apparatus. The cellular role of OCRL1 is unknown, and consequently the mechanism by which loss of OCRL1 function leads to disease is ill defined. Here, we show that OCRL1 is associated with clathrin-coated transport intermediates operating between the TGN and endosomes. OCRL1 interacts directly with clathrin heavy chain and promotes clathrin assembly in vitro. Interaction with clathrin is not, however, required for membrane association of OCRL1. Overexpression of OCRL1 results in redistribution of clathrin and the cation-independent mannose 6-phosphate receptor (CI-MPR) to enlarged endosomal structures that are defective in retrograde trafficking to the TGN. Depletion of cellular OCRL1 also causes a partial redistribution of a CI-MPR reporter to early endosomes. These findings suggest a role for OCRL1 in clathrin-mediated trafficking of proteins from endosomes to the TGN, and that defects in this pathway might contribute to the Lowe syndrome phenotype.

Address correspondence to: Martin Lowe (lowe{at}man.ac.uk)




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
R. Choudhury, C. J. Noakes, E. McKenzie, C. Kox, and M. Lowe
Differential Clathrin Binding and Subcellular Localization of OCRL1 Splice Isoforms
J. Biol. Chem., April 10, 2009; 284(15): 9965 - 9973.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
H. J. McCrea and P. De Camilli
Mutations in Phosphoinositide Metabolizing Enzymes and Human Disease
Physiology, February 1, 2009; 24(1): 8 - 16.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
S. S. Deepa and L. Q. Dong
APPL1: role in adiponectin signaling and beyond
Am J Physiol Endocrinol Metab, January 1, 2009; 296(1): E22 - E36.
[Abstract] [Full Text] [PDF]

Home page
 CJASNHome page
D. Bockenhauer, A. Bokenkamp, W. van't Hoff, E. Levtchenko, J. E. Kist-van Holthe, V. Tasic, and M. Ludwig
Renal Phenotype in Lowe Syndrome: A Selective Proximal Tubular Dysfunction
Clin. J. Am. Soc. Nephrol., September 1, 2008; 3(5): 1430 - 1436.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
J. Wright, M. M. Morales, J. Sousa-Menzes, D. Ornellas, J. Sipes, Y. Cui, I. Cui, P. Hulamm, V. Cebotaru, L. Cebotaru, et al.
Transcriptional adaptation to Clcn5 knockout in proximal tubules of mouse kidney
Physiol Genomics, May 1, 2008; 33(3): 341 - 354.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. Williams, R. Choudhury, E. McKenzie, and M. Lowe
Targeting of the type II inositol polyphosphate 5-phosphatase INPP5B to the early secretory pathway
J. Cell Sci., November 15, 2007; 120(22): 3941 - 3951.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. Vilasi, P. R. Cutillas, A. D. Maher, S. F. M. Zirah, G. Capasso, A. W. G. Norden, E. Holmes, J. K. Nicholson, and R. J. Unwin
Combined proteomic and metabonomic studies in three genetic forms of the renal Fanconi syndrome
Am J Physiol Renal Physiol, August 1, 2007; 293(2): F456 - F467.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
E. Fuchs, A. K. Haas, R. A. Spooner, S.-i. Yoshimura, J. M. Lord, and F. A. Barr
Specific Rab GTPase-activating proteins define the Shiga toxin and epidermal growth factor uptake pathways
J. Cell Biol., July 30, 2007; 177(6): 1133 - 1143.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. Mukhopadhyay, F. Xu, and P. B. Sehgal
Aberrant cytoplasmic sequestration of eNOS in endothelial cells after monocrotaline, hypoxia, and senescence: live-cell caveolar and cytoplasmic NO imaging
Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1373 - H1389.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
V. M. Olkkonen and E. Ikonen
When intracellular logistics fails - genetic defects in membrane trafficking
J. Cell Sci., December 15, 2006; 119(24): 5031 - 5045.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. J. Guerriero, K. M. Weixel, J. R. Bruns, and O. A. Weisz
Phosphatidylinositol 5-Kinase Stimulates Apical Biosynthetic Delivery via an Arp2/3-dependent Mechanism
J. Biol. Chem., June 2, 2006; 281(22): 15376 - 15384.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
H.-W. Shin, M. Hayashi, S. Christoforidis, S. Lacas-Gervais, S. Hoepfner, M. R. Wenk, J. Modregger, S. Uttenweiler-Joseph, M. Wilm, A. Nystuen, et al.
An enzymatic cascade of Rab5 effectors regulates phosphoinositide turnover in the endocytic pathway
J. Cell Biol., August 15, 2005; 170(4): 607 - 618.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.