Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print July 19, 2005
Mol. Biol. Cell 10.1091/mbc.E05-03-0243

A more recent version of this article appeared on October 1, 2005
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E05-03-0243v1
16/10/4543    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Knop, M.
Right arrow Articles by Jäntti, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Knop, M.
Right arrow Articles by Jäntti, J.

Submitted on March 22, 2005
Revised on May 25, 2005
Accepted on July 7, 2005

Molecular Interactions Position Mso1p, a Novel PTB Domain Homologue, in the Interface of the Exocyst Complex and the Exocytic SNARE Machinery in Yeast

Michael Knop,* K. Juha Miller,{dagger} Massimiliano Mazza,* DeJiang Feng,{dagger}{ddagger} Marion Weber,{ddagger} Sirkka Keränen,{dagger} and Jussi Jäntti{dagger}{ddagger}

*EMBL, Heidelberg D-69117, Germany; {dagger}VTT Biotechnology, FIN-02044 VTT, Finland; {ddagger}Institute of Biotechnology, University of Helsinki, 00014 University of Helsinki, Finland

Monitoring Editor: Jennifer Lippincott-Schwartz

In this study we have analyzed the association of the Sec1p interacting protein Mso1p with the membrane fusion machinery in yeast. We show that Mso1p is essential for vesicle fusion during prospore membrane formation. GFP-tagged Mso1p localizes to the sites of exocytosis, and at the site of prospore membrane formation. In vivo and in vitro experiments identified a short amino-terminal sequence in Mso1p that mediates its interaction with Sec1p and is needed for vesicle fusion. A point mutation, T47A, within the Sec1p-binding domain abolishes Mso1p functionality in vivo and mso1T47A mutant cells display specific genetic interactions with sec1 mutants. Mso1p coimmunoprecipitates with Sec1p, Sso1/2p, Snc1/2p, Sec9p, and the exocyst complex subunit Sec15p. In sec4-8 and SEC4I133 mutant cells association of Mso1p with Sso1/2p, Snc1/2p, and Sec9p is affected whereas interaction with Sec1p persists. Furthermore, in SEC4I133 cells the dominant negative Sec4I133p coimmunoprecipitates with Mso1p-Sec1p complex. Finally, we identify Mso1p as a homologue of the PTB binding domain of the mammalian Sec1p binding Mint proteins. These results position Mso1p in the interface of the exocyst complex, Sec4p, and the SNARE machinery, and reveal a novel layer of molecular conservation in the exocytosis machinery.

Address correspondence to: Michael Knop (knop{at}embl.de) or Jussi Jäntti (jussi.jantti{at}helsinki.fi)




This article has been cited by other articles:


Home page
 Eukaryot CellHome page
Y. Suda, H. Nakanishi, E. M. Mathieson, and A. M. Neiman
Alternative Modes of Organellar Segregation during Sporulation in Saccharomyces cerevisiae
Eukaryot. Cell, November 1, 2007; 6(11): 2009 - 2017.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. Nakanishi, Y. Suda, and A. M. Neiman
Erv14 family cargo receptors are necessary for ER exit during sporulation in Saccharomyces cerevisiae
J. Cell Sci., March 1, 2007; 120(5): 908 - 916.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. Nakanishi, M. Morishita, C. L. Schwartz, A. Coluccio, J. Engebrecht, and A. M. Neiman
Phospholipase D and the SNARE Sso1p are necessary for vesicle fusion during sporulation in yeast.
J. Cell Sci., April 1, 2006; 119(Pt 7): 1406 - 1415.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
C. Taxis, P. Keller, Z. Kavagiou, L. J. Jensen, J. Colombelli, P. Bork, E. H.K. Stelzer, and M. Knop
Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system
J. Cell Biol., November 21, 2005; 171(4): 627 - 640.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. G. Riedel, M. Mazza, P. Maier, R. Korner, and M. Knop
Differential Requirement for Phospholipase D/Spo14 and Its Novel Interactor Sma1 for Regulation of Exocytotic Vesicle Fusion in Yeast Meiosis
J. Biol. Chem., November 11, 2005; 280(45): 37846 - 37852.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.