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MBC in Press, published online ahead of print January 11, 2006
Mol. Biol. Cell 10.1091/mbc.E05-04-0271

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Submitted on April 1, 2005
Revised on December 5, 2005
Accepted on January 3, 2006

Chromokinesin Xklp1 Contributes to the Regulation of Microtubule Density and Organization during Spindle Assembly

Mirco Castoldi and Isabelle Vernos

Cell Biology and Biophysics Program, EMBL, 69117 Heidelberg, Germany

Monitoring Editor: Erika Holzbaur

Xklp1 is a chromosome-associated kinesin required for Xenopus early embryonic cell division. Function blocking experiments in Xenopus egg extracts suggested that it is required for spindle assembly. We have reinvestigated Xklp1 function(s) by monitoring spindle assembly and microtubule behavior under a range of Xklp1 concentrations in egg extracts. We found that in the absence of Xklp1, bipolar spindles form with a reduced efficiency and display abnormalities associated with an increased microtubule mass. Likewise, centrosomal asters assembled in Xklp1-depleted extract show an increased microtubule mass. Conversely, addition of recombinant Xklp1 to the extract reduces the microtubule mass associated with spindles and asters. Our data suggest that Xklp1 affects microtubule polymerization during M-phase. We propose that these attributes, combined with Xklp1 plus-end directed motility, contribute to the assembly of a functional bipolar spindle.

Address correspondence to: Isabelle Vernos (isabelle.vernos{at}crg.es)




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