Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print August 17, 2005
Mol. Biol. Cell 10.1091/mbc.E05-04-0338

A more recent version of this article appeared on November 1, 2005
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Movies
Right arrow All Versions of this Article:
E05-04-0338v1
16/11/5127    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Siller, K. H.
Right arrow Articles by Doe, C. Q.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Siller, K. H.
Right arrow Articles by Doe, C. Q.

Submitted on April 22, 2005
Revised on July 8, 2005
Accepted on August 10, 2005

Live Imaging of Drosophila Brain Neuroblasts Reveals a Role for Lis1/Dynactin in Spindle Assembly and Mitotic Checkpoint Control

Karsten H. Siller,* Madeline Serr,{dagger} Ruth Steward,{ddagger} Tom S. Hays,{dagger} and Chris Q. Doe*

*Howard Hughes Medical Institute, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403; {dagger}Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455; {ddagger}Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08854

Monitoring Editor: Erika Holzbaur

Lis1 is required for nuclear migration in fungi, cell cycle progression in mammals, and the formation of a folded cerebral cortex in humans. Lis1 binds dynactin and the dynein motor complex, but the role of Lis1 in many dynein/dynactin-dependent processes is not clearly understood. Here we generate and/or characterize mutants for Drosophila Lis1 and a dynactin subunit, Glued, to investigate the role of Lis1/dynactin in mitotic checkpoint function. In addition, we develop an improved time-lapse video microscopy technique that allows live imaging of GFP-Lis1, GFP-Rod checkpoint protein, GFP-labeled chromosomes, or GFP-labeled mitotic spindle dynamics in neuroblasts within whole larval brain explants. Our mutant analyses show that Lis1/dynactin have at least two independent functions during mitosis: first promoting centrosome separation and bipolar spindle assembly during prophase/prometaphase, and subsequently generating interkinetochore tension and transporting checkpoint proteins off kinetochores during metaphase, thus promoting timely anaphase onset. Furthermore, we show that Lis1/dynactin/dynein physically associate and colocalize on centrosomes, spindle MTs, and kinetochores, and that regulation of Lis1/dynactin kinetochore localization in Drosophila differs from both C. elegans and mammals. We conclude that Lis1/dynactin act together to regulate multiple, independent functions in mitotic cells, including spindle formation and cell cycle checkpoint release.

Address correspondence to: Chris Q. Doe (cdoe{at}uoneuro.uoregon.edu)




This article has been cited by other articles:


Home page
 JCBHome page
J. Januschke and C. Gonzalez
The interphase microtubule aster is a determinant of asymmetric division orientation in Drosophila neuroblasts
J. Cell Biol., March 8, 2010; 188(5): 693 - 706.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. Lam, M. A. S. Vergnolle, L. Thorpe, P. G. Woodman, and V. J. Allan
Functional interplay between LIS1, NDE1 and NDEL1 in dynein-dependent organelle positioning
J. Cell Sci., January 15, 2010; 123(2): 202 - 212.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
R. A. Neumuller and J. A. Knoblich
Dividing cellular asymmetry: asymmetric cell division and its implications for stem cells and cancer
Genes & Dev., December 1, 2009; 23(23): 2675 - 2699.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
Z. Rahmani, M. E. Gagou, C. Lefebvre, D. Emre, and R. E. Karess
Separating the spindle, checkpoint, and timer functions of BubR1
J. Cell Biol., November 30, 2009; 187(5): 597 - 605.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. Reis, T. Feijao, S. Gouveia, A. J. Pereira, I. Matos, P. Sampaio, H. Maiato, and C. E. Sunkel
Dynein and Mast/Orbit/CLASP have antagonistic roles in regulating kinetochore-microtubule plus-end dynamics
J. Cell Sci., July 15, 2009; 122(14): 2543 - 2553.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Wainman, J. Creque, B. Williams, E. V. Williams, S. Bonaccorsi, M. Gatti, and M. L. Goldberg
Roles of the Drosophila NudE protein in kinetochore function and centrosome migration
J. Cell Sci., June 1, 2009; 122(11): 1747 - 1758.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
N. M. Rusan, K. Akong, and M. Peifer
Putting the model to the test: are APC proteins essential for neuronal polarity, axon outgrowth, and axon targeting?
J. Cell Biol., October 20, 2008; 183(2): 203 - 212.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. C. Stone, F. Roegiers, and M. M. Rolls
Microtubules Have Opposite Orientation in Axons and Dendrites of Drosophila Neurons
Mol. Biol. Cell, October 1, 2008; 19(10): 4122 - 4129.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A. S. Pawlisz, C. Mutch, A. Wynshaw-Boris, A. Chenn, C. A. Walsh, and Y. Feng
Lis1-Nde1-dependent neuronal fate control determines cerebral cortical size and lamination
Hum. Mol. Genet., August 15, 2008; 17(16): 2441 - 2455.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. Chabu and C. Q. Doe
Dap160/intersectin binds and activates aPKC to regulate cell polarity and cell cycle progression
Development, August 15, 2008; 135(16): 2739 - 2746.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
Y. M. Yamashita and M. T. Fuller
Asymmetric centrosome behavior and the mechanisms of stem cell division
J. Cell Biol., January 28, 2008; 180(2): 261 - 266.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. Zhang and T. L. Megraw
Proper Recruitment of {gamma}-Tubulin and D-TACC/Msps to Embryonic Drosophila Centrosomes Requires Centrosomin Motif 1
Mol. Biol. Cell, October 1, 2007; 18(10): 4037 - 4049.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. W. Nipper, K. H. Siller, N. R. Smith, C. Q. Doe, and K. E. Prehoda
G{alpha}i generates multiple Pins activation states to link cortical polarity and spindle orientation in Drosophila neuroblasts
PNAS, September 4, 2007; 104(36): 14306 - 14311.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
E. R. Griffis, N. Stuurman, and R. D. Vale
Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore
J. Cell Biol., July 30, 2007; 177(6): 1005 - 1015.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
N. M. Rusan and M. Peifer
A role for a novel centrosome cycle in asymmetric cell division
J. Cell Biol., April 9, 2007; 177(1): 13 - 20.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
C.-Y. Lee, R. O. Andersen, C. Cabernard, L. Manning, K. D. Tran, M. J. Lanskey, A. Bashirullah, and C. Q. Doe
Drosophila Aurora-A kinase inhibits neuroblast self-renewal by regulating aPKC/Numb cortical polarity and spindle orientation
Genes & Dev., December 15, 2006; 20(24): 3464 - 3474.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J.-G. Delcros, C. Prigent, and R. Giet
Dynactin targets Pavarotti-KLP to the central spindle during anaphase and facilitates cytokinesis in Drosophila S2 cells
J. Cell Sci., November 1, 2006; 119(21): 4431 - 4441.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
D. J. Solecki, E.-E. Govek, T. Tomoda, and M. E. Hatten
Neuronal polarity in CNS development
Genes & Dev., October 1, 2006; 20(19): 2639 - 2647.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.