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MBC in Press, published online ahead of print November 16, 2005
Mol. Biol. Cell 10.1091/mbc.E05-04-0360

A more recent version of this article appeared on February 1, 2006
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Submitted on April 28, 2005
Revised on November 7, 2005
Accepted on November 8, 2005

Nudel Contributes to MT Anchoring at the Mother Centriole and Is Involved in Both Dynein-dependent and -independent Centrosomal Protein Assembly

Jing Guo, Zhenye Yang, Wei Song, Qi Chen, Fubin Wang, Qiangge Zhang, and Xueliang Zhu

Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, and Graduate School, Chinese Academy of Sciences, Shanghai 200031, China

Monitoring Editor: Kerry Bloom

The centrosome is the major MT-organizing center in animal cells. Although the cytoplasmic dynein regulator Nudel interacts with centrosomes, its role herein remains unclear. Here we show that in Cos7 cells Nudel is a mother centriole protein with rapid turnover independent of dynein activity. During centriole duplication, Nudel targets to the new mother centriole later than ninein but earlier than dynactin. Its centrosome localization requires a C-terminal region that is essential for associations with dynein, dynactin, PCM-1, pericentrin, and {gamma}-tubulin. Overexpression of a mutant Nudel lacking this region, a treatment previously shown to inactivate dynein, dislocates centrosomal Lis1, dynactin, and PCM-1, with little influence on pericentrin and {gamma}-tubulin in Cos7 and HeLa cells. Silencing Nudel in HeLa cells markedly decreases centrosomal targeting of all the aforementioned proteins. Silencing Nudel also represses centrosomal MT nucleation and anchoring. Furthermore, Nudel can interact with pericentrin independently of dynein. Our current results suggest that Nudel plays a role in both dynein-mediated centripetal transport of dynactin, Lis1, and PCM-1 as well as in dynein-independent centrosomal targeting of pericentrin and {gamma}-tubulin. Moreover, Nudel appears to tether dynactin and dynein to the mother centriole for MT anchoring.

Address correspondence to: Xueliang Zhu (xlzhu{at}sibs.ac.cn)




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