Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print July 19, 2005
Mol. Biol. Cell 10.1091/mbc.E05-05-0426

A more recent version of this article appeared on October 1, 2005
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E05-05-0426v1
16/10/4954    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lu, H.
Right arrow Articles by Yamaoka, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lu, H.
Right arrow Articles by Yamaoka, Y.

Submitted on May 16, 2005
Revised on June 24, 2005
Accepted on July 13, 2005

Regulation of Interleukin-6 Promoter Activation in Gastric Epithelial Cells Infected with Helicobacter pylori

Hong Lu,* Jeng Yih Wu,* Takahiko Kudo,* Tomoyuki Ohno,{dagger} David Y. Graham,* and Yoshio Yamaoka*

*Department of Medicine, Michael E. DeBakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, TX 77030; {dagger}Department of Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Kyoto 602-0841, Japan

Monitoring Editor: J. Silvio Gutkind

The regulation of Helicobacter pylori induced interleukin (IL)-6 in the gastric epithelium remains unclear. Primary gastric epithelial cells and MKN28 cells were cocultured with H. pylori and its isogenic cag pathogenicity island (PAI) mutant and/or oipA mutants. H. pylori infection induced IL-6 mRNA expression and IL-6 protein production which was further enhanced by the cag PAI and OipA. Luciferase reporter gene assays and electrophoretic mobility shift assays showed that full IL-6 transcription required binding sites for nuclear factor-{kappa}B (NF-{kappa}B), cAMP response element (CRE), CCAAT/enhancer binding protein (C/EBP) and activator protein (AP)-1. The cag PAI and OipA were involved in binding to NF-{kappa}B, AP-1, CRE and C/EBP sites. The cag PAI activated the extracellular signal-regulated kinase (ERK) and Jun N-terminal kinase (JNK) pathways; OipA activated the p38 pathway. Transfection of dominant negative G-protein confirmed roles for Raf, Rac1 and RhoA in IL-6 induction. Overall, the cag PAI-related IL-6 signal transduction pathway involved Ras/Raf/MEK1/2/ERK/AP-1/CRE pathway and JNK/AP-1/CRE pathway, OipA-related pathway is p38/AP-1/CRE and both the cag PAI and OipA appear to be involved in the RhoA/Rac1/NF-{kappa}B pathway. Combination of different pathways by the cag PAI and OipA will lead the maximum IL-6 induction.

Address correspondence to: Yoshio Yamaoka (yyamaoka{at}bcm.tmc.edu)




This article has been cited by other articles:


Home page
 J. Immunol.Home page
J. Hisatsune, M. Nakayama, H. Isomoto, H. Kurazono, N. Mukaida, A. K. Mukhopadhyay, T. Azuma, Y. Yamaoka, J. Sap, E. Yamasaki, et al.
Molecular Characterization of Helicobacter pylori VacA Induction of IL-8 in U937 Cells Reveals a Prominent Role for p38MAPK in Activating Transcription Factor-2, cAMP Response Element Binding Protein, and NF-{kappa}B Activation
J. Immunol., April 1, 2008; 180(7): 5017 - 5027.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. T. Franco, E. Johnston, U. Krishna, Y. Yamaoka, D. A. Israel, T. A. Nagy, L. E. Wroblewski, M. B. Piazuelo, P. Correa, and R. M. Peek Jr.
Regulation of Gastric Carcinogenesis by Helicobacter pylori Virulence Factors
Cancer Res., January 15, 2008; 68(2): 379 - 387.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Yamauchi, I.-J. Choi, H. Lu, H. Ogiwara, D. Y. Graham, and Y. Yamaoka
Regulation of IL-18 in Helicobacter pylori Infection
J. Immunol., January 15, 2008; 180(2): 1207 - 1216.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. H. Pillinger, N. Marjanovic, S.-Y. Kim, Y.-C. Lee, J. U. Scher, J. Roper, A. M. Abeles, P. I. Izmirly, M. Axelrod, M. Y. Pillinger, et al.
Helicobacter pylori Stimulates Gastric Epithelial Cell MMP-1 Secretion via CagA-dependent and -independent ERK Activation
J. Biol. Chem., June 29, 2007; 282(26): 18722 - 18731.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Lu, J. Y. Wu, E. J. Beswick, T. Ohno, S. Odenbreit, R. Haas, V. E. Reyes, M. Kita, D. Y. Graham, and Y. Yamaoka
Functional and Intracellular Signaling Differences Associated with the Helicobacter pylori AlpAB Adhesin from Western and East Asian Strains
J. Biol. Chem., March 2, 2007; 282(9): 6242 - 6254.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Y. Wu, H. Lu, Y. Sun, D. Y. Graham, H. S. Cheung, and Y. Yamaoka
Balance between Polyoma Enhancing Activator 3 and Activator Protein 1 Regulates Helicobacter pylori-Stimulated Matrix Metalloproteinase 1 Expression.
Cancer Res., May 15, 2006; 66(10): 5111 - 5120.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.