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A more recent version of this article appeared on January 1, 2006
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Submitted on May 23, 2005
Revised on September 30, 2005
Accepted on October 14, 2005
Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, CO 80045
Monitoring Editor: Benjamin Glick
The role of cis-medial Golgi matrix proteins in retrograde traffic is poorly understood. We have used imaging techniques to understand the relationship between the cis-medial Golgi matrix and transmembrane proteins during retrograde traffic in control and Brefeldin A (BFA) treated cells. All five of the cis-medial matrix proteins tested were associated with retrograde tubules within 2-3 min of initiation of tubule formation. Then at later time points (3-10 min) transmembrane proteins are apparent in the same tubules. Strikingly, both the matrix proteins and the transmembrane proteins moved directly to ER exit sites labeled with p58 and Sec13 and there appeared to be a specific interaction between the ER exit sites and the tips or branch points of the tubules enriched for the matrix proteins. After the initial interaction Golgi matrix proteins accumulated rapidly (5-10 min) at ER exit sites and Golgi transmembrane proteins accumulated at the same sites 
2 h later. Our data suggests that Golgi cis-medial matrix proteins participate in Golgi to ER traffic and play a novel role in tubule formation and targeting.
Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239.
Address correspondence to:
Kathryn E. Howell (kathryn.howell{at}uchsc.edu)
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