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A more recent version of this article appeared on January 1, 2006
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Submitted on June 7, 2005
Revised on September 27, 2005
Accepted on October 24, 2005
*State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, SIBS, CAS, Shanghai 200031, China; Department of Biological Chemistry, College of Medicine, University of California, Irvine, Irvine, CA 92697-1700
Monitoring Editor: Tim Stearns
The transcription factor Flo8 is essential for filamentous growth in S. cerevisiae and is regulated under the cAMP/protein kinase A (PKA) pathway. To determine whether a similar pathway/regulation exists in Candida albicans, we have cloned C. albicans FLO8 by its ability to complement S. cerevisiae flo8. Deleting FLO8 in C. albicans blocked hyphal development and hypha-specific gene expression. The flo8/flo8 mutant is avirulent in a mouse model of systemic infection. Genome-wide transcription profiling of efg1/efg1 and flo8/flo8 using a C. albicans DNA microarray suggests that Flo8 controls subsets of Efg1-regulated genes. Most of these genes are hypha-specific, including HGC1 and IHD1. We also show that Flo8 interacts with Efg1 in yeast and hyphal cells by in vivo immunoprecipitation. Similar to efg1/efg1, flo8/flo8 and cdc35/cdc35 show enhanced hyphal growth under an embedded growth condition. Our results suggest that Flo8 may function downstream of the cAMP/PKA pathway, and together with Efg1, regulates the expression of hypha-specific genes and genes that are important for the virulence of C. albicans.
These authors contributed equally to this work.
Address correspondence to:
Jiangye Chen (jychen{at}sunm.shcnc.ac.cn) or Haoping Liu (h4liu{at}uci.edu)
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