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MBC in Press, published online ahead of print December 7, 2005
Mol. Biol. Cell 10.1091/mbc.E05-06-0542

A more recent version of this article appeared on February 1, 2006
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Submitted on June 17, 2005
Revised on November 28, 2005
Accepted on November 29, 2005

Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo

Anne Straube,*{dagger} Gerd Hause,{ddagger} Gero Fink,* and Gero Steinberg*

*Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg, Germany; {dagger}Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland, United Kingdom; {ddagger}Martin-Luther-Universität Halle-Wittenberg, Biozentrum, D-06099 Halle, Germany

Monitoring Editor: J. Richard McIntosh

Conventional kinesin is an ubiquitous organelle transporter that moves cargo toward the plus ends of microtubules. In addition, several in vitro studies indicated a role of conventional kinesin in cross-bridging and sliding microtubules, but in vivo evidence for such a role is missing. In this study we show that conventional kinesin mediates microtubule-microtubule interactions in the model fungus Ustilago maydis. Live cell imaging and ultrastructural analysis of various mutants in Kin1, revealed that this Kinesin-1 motor is required for efficient microtubule bundling and participates in microtubule bending in vivo. High levels of Kin1 led to increased microtubule bending, while a rigor-mutation in the motor head suppressed all microtubule motility and promoted strong microtubule bundling, indicating that kinesin can form cross-bridges between microtubules in living cells. This effect required a conserved region in the C-terminus of Kin1, which was shown to bind microtubules in vitro. In addition, a fusion protein of YFP and the Kin1tail localized to microtubule bundles, further supporting the idea that a conserved microtubule binding activity in the tail of conventional kinesins mediates microtubule-microtubule interactions in vivo.

Address correspondence to: Gero Steinberg (gero.steinberg{at}staff.uni-marburg.de)




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