Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print December 7, 2005
Mol. Biol. Cell 10.1091/mbc.E05-07-0647

A more recent version of this article appeared on February 1, 2006
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E05-07-0647v1
17/2/966    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sakurai, A.
Right arrow Articles by Mochizuki, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sakurai, A.
Right arrow Articles by Mochizuki, N.

Submitted on July 19, 2005
Revised on November 28, 2005
Accepted on November 29, 2005

MAGI-1 Is Required for Rap1 Activation upon Cell-Cell Contact and For Enhancement of Vascular Endothelial Cadherin-mediated Cell Adhesion

Atsuko Sakurai, Shigetomo Fukuhara, Akiko Yamagishi, Keisuke Sako, Yuji Kamioka, Michitaka Masuda, Yoshikazu Nakaoka, and Naoki Mochizuki

Department of Structural Analysis, National Cardiovascular Center Research Institute, Suita, Osaka 565-8565, Japan

Monitoring Editor: Martin A. Schwartz

Rap1 is a small GTPase which regulates adherens junction maturation. It remains elusive how Rap1 is activated upon cell-cell contact. We demonstrate for the first time that Rap1 is activated upon homophilic engagement of vascular endothelial cadherin (VE-cadherin) at the cell-cell contacts in living cells and that MAGI-1 is required for VE-cadherin-dependent Rap1 activation. We found that MAGI-1 localized to cell-cell contacts presumably by associating with {beta}-catenin and that MAGI-1 bound to a guanine nucleotide exchange factor for Rap1, PDZ-GEF1. Depletion of MAGI-1 suppressed the cell-cell contact-induced Rap1 activation and the VE-cadherin-mediated cell-cell adhesion after Ca2+ switch. In addition, relocation of vinculin from cell-extracellular matrix contacts to cell-cell contacts after the Ca2+ switch was inhibited in MAGI-1-depleted cells. Furthermore, inactivation of Rap1 by overexpression of Rap1GAPII impaired the VE-cadherin-dependent cell adhesion. Collectively, MAGI-1 is important for VE-cadherin-dependent Rap1 activation upon cell-cell contact. In addition, once activated Rap1 upon cell-cell contacts positively regulate the adherens junction formation by relocating vinculin that supports VE-cadherin-based cell adhesion.

Address correspondence to: Naoki Mochizuki (nmochizu{at}ri.ncvc.go.jp)




This article has been cited by other articles:


Home page
 J. Neurosci.Home page
Z. Xu, A. W. Peng, K. Oshima, and S. Heller
MAGI-1, A Candidate Stereociliary Scaffolding Protein, Associates with the Tip-Link Component Cadherin 23
J. Neurosci., October 29, 2008; 28(44): 11269 - 11276.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. Guillemot, S. Paschoud, L. Jond, A. Foglia, and S. Citi
Paracingulin Regulates the Activity of Rac1 and RhoA GTPases by Recruiting Tiam1 and GEF-H1 to Epithelial Junctions
Mol. Biol. Cell, October 1, 2008; 19(10): 4442 - 4453.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. Dejana, F. Orsenigo, and M. G. Lampugnani
The role of adherens junctions and VE-cadherin in the control of vascular permeability
J. Cell Sci., July 1, 2008; 121(13): 2115 - 2122.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
D. D. Mruk, B. Silvestrini, and C. Y. Cheng
Anchoring Junctions As Drug Targets: Role in Contraceptive Development
Pharmacol. Rev., June 1, 2008; 60(2): 146 - 180.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
D. Vestweber
VE-Cadherin: The Major Endothelial Adhesion Molecule Controlling Cellular Junctions and Blood Vessel Formation
Arterioscler. Thromb. Vasc. Biol., February 1, 2008; 28(2): 223 - 232.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. E. Zemljic-Harpf, J. C. Miller, S. A. Henderson, A. T. Wright, A. M. Manso, L. Elsherif, N. D. Dalton, A. K. Thor, G. A. Perkins, A. D. McCulloch, et al.
Cardiac-Myocyte-Specific Excision of the Vinculin Gene Disrupts Cellular Junctions, Causing Sudden Death or Dilated Cardiomyopathy
Mol. Cell. Biol., November 1, 2007; 27(21): 7522 - 7537.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. J. Lorenowicz, M. Fernandez-Borja, and P. L. Hordijk
cAMP Signaling in Leukocyte Transendothelial Migration
Arterioscler. Thromb. Vasc. Biol., May 1, 2007; 27(5): 1014 - 1022.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
E. Aivatiadou, E. Mattei, M. Ceriani, L. Tilia, and G. Berruti
Impaired Fertility and Spermiogenetic Disorders with Loss of Cell Adhesion in Male Mice Expressing an Interfering Rap1 Mutant
Mol. Biol. Cell, April 1, 2007; 18(4): 1530 - 1542.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. M. Shasby
Cell-cell adhesion in lung endothelium
Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L593 - L607.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. R. H. Kooistra, N. Dube, and J. L. Bos
Rap1: a key regulator in cell-cell junction formation
J. Cell Sci., January 1, 2007; 120(1): 17 - 22.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
A. Kawata, J. Iida, M. Ikeda, Y. Sato, H. Mori, A. Kansaku, K. Sumita, N. Fujiwara, C. Rokukawa, M. Hamano, et al.
CIN85 Is Localized at Synapses and Forms a Complex with S-SCAM via Dendrin.
J. Biochem., May 1, 2006; 139(5): 931 - 939.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.