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MBC in Press, published online ahead of print December 7, 2005
Mol. Biol. Cell 10.1091/mbc.E05-08-0704

A more recent version of this article appeared on February 1, 2006
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Submitted on August 1, 2005
Revised on November 21, 2005
Accepted on November 29, 2005

Contractile Ring-independent Localization of DdINCENP, a Protein Important for Spindle Stability and Cytokinesis

Qian Chen, Hui Li, and Arturo De Lozanne

Institute of Cellular and Molecular Biology and Department of Molecular, Cellular, and Developmental Biology, University of Texas at Austin, Austin, TX 78712

Monitoring Editor: J. Richard McIntosh

Dictyostelium DdINCENP is a chromosomal passenger protein associated with centromeres, the spindle midzone and poles during mitosis and the cleavage furrow during cytokinesis. Disruption of the single DdINCENP gene revealed important roles for this protein in mitosis and cytokinesis. DdINCENP null cells lack a robust spindle midzone and are hypersensitive to microtubule depolymerizing drugs suggesting that their spindles may not be stable. Furthermore DdCP224, a protein homologous to the microtubule-stabilizing protein TOGp/XMAP215, was absent from the spindle midzone of DdINCENP null cells. Overexpression of DdCP224 rescued the weak spindle midzone defect of DdINCENP null cells. While not required for the localization of the myosin II contractile ring and subsequent formation of a cleavage furrow, DdINCENP is important for the abscission of daughter cells at the end of cytokinesis. Finally, we show that the localization of DdINCENP at the cleavage furrow is modulated by myosin II but it occurs by a mechanism different from that controlling the formation of the contractile ring.

Address correspondence to: Arturo De Lozanne (a.delozanne{at}mail.utexas.edu)




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