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MBC in Press, published online ahead of print September 29, 2005
Mol. Biol. Cell 10.1091/mbc.E05-08-0715

A more recent version of this article appeared on December 1, 2005
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Submitted on August 3, 2005
Accepted on September 15, 2005

Nup153 Affects Entry of Messenger and Ribosomal RNPs into the Nuclear Basket during Export

Teresa Soop, Birgitta Ivarsson, Birgitta Björkroth, Nathalie Fomproix, Sergej Masich, Volker C. Cordes,* and Bertil Daneholt

Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, S-171 77 Stockholm, Sweden

Monitoring Editor: Joseph Gall

A specific messenger RNP particle, Balbiani ring (BR) granules in the dipteran Chironomus tentans, can be visualized during passage through the nuclear pore complex (NPC). We have now examined the transport through the nuclear basket preceding the actual translocation through NPC. The basket consists of eight fibrils anchored to the NPC core by nucleoprotein Nup153. On nuclear injection of anti-Nup153, the transport of BR granules is blocked. Many granules are retained on top of the nuclear basket, while no granules are seen in transit through NPC. Interestingly, the effect of Nup153 appears distant from the antibody-binding site at the base of the basket. We conclude that the entry into the basket is a two-step process: an mRMP first binds to the tip of the basket fibrils and only then is it transferred into the basket by a Nup153-dependent process. It is indicated that ribosomal subunits follow a similar pathway.

*Present address: Zentrum für Molekulare Biologie, University of Heidelberg, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany.

Address correspondence to: Bertil Daneholt (bertil.daneholt{at}cmb.ki.se)




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