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MBC in Press, published online ahead of print December 21, 2005
Mol. Biol. Cell 10.1091/mbc.E05-08-0768

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Submitted on August 17, 2005
Revised on November 30, 2005
Accepted on December 2, 2005

Dynamic Nature of Cleavage Bodies and their Spatial Relationship to DDX1 Bodies, Cajal Bodies, and Gems

Lei Li, Ken Roy, Sachin Katyal, Xuejun Sun, Stacey Bléoo, and Roseline Godbout

Department of Oncology, University of Alberta, Cross Cancer Institute, Edmonton, Alberta T6G 1Z2, Canada

Monitoring Editor: Joseph Gall

DDX1 bodies, cleavage bodies, Cajal bodies (CBs) and gems are nuclear suborganelles that contain factors involved in RNA transcription and/or processing. Although all four nuclear bodies can exist as distinct entities, they often colocalize or overlap with each other. To better understand the relationship between these four nuclear bodies, we examined their spatial distribution as a function of the cell cycle. Here, we report that whereas DDX1 bodies, CBs and gems are present throughout interphase, CPSF-100-containing cleavage bodies are predominantly found during S and G2 phases, while CstF-64-containing cleavage bodies are primarily observed during S phase. All four nuclear bodies associate with each other during S phase, with cleavage bodies colocalizing with DDX1 bodies, and cleavage bodies/DDX1 bodies residing adjacent to gems and CBs. While inhibitors of RNA transcription had no effect on DDX1 bodies or cleavage bodies, inhibitors of DNA replication resulted in loss of CstF-64-containing cleavage bodies. A striking effect on nuclear structures was observed with latrunculin B, an inhibitor of actin polymerization, resulting in the formation of needle-like nuclear spicules made up of CstF-64, CPSF-100, RNA and RNA polymerase II. Our results suggest that cleavage body components are highly dynamic in nature.

Address correspondence to: Roseline Godbout (rgodbout{at}ualberta.ca)




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L. Li, E. A. Monckton, and R. Godbout
A Role for DEAD Box 1 at DNA Double-Strand Breaks
Mol. Cell. Biol., October 15, 2008; 28(20): 6413 - 6425.
[Abstract] [Full Text] [PDF]


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