Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print January 18, 2006
Mol. Biol. Cell 10.1091/mbc.E05-08-0792

A more recent version of this article appeared on April 1, 2006
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E05-08-0792v1
17/4/1514    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sannerud, R.
Right arrow Articles by Saraste, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sannerud, R.
Right arrow Articles by Saraste, J.

Submitted on August 25, 2005
Revised on December 21, 2005
Accepted on January 5, 2006

Rab1 Defines a Novel Pathway Connecting the Pre-Golgi Intermediate Compartment with the Cell Periphery

Ragna Sannerud,* Michaël Marie,* Clément Nizak,{dagger}{ddagger} Hege Avsnes Dale,{sect} Karin Pernet-Gallay,{dagger} Franck Perez,{dagger} Bruno Goud,{dagger} and Jaakko Saraste*

*Section of Anatomy and Cell Biology and {sect}Molecular Imaging Center, Department of Biomedicine, University of Bergen, N-5009 Bergen, Norway; {dagger}CNRS UMR 144, Institut Curie, Section de Recherche, 75248 Paris Cedex 05, France

Monitoring Editor: Jennifer Lippincott-Schwartz

The function of the pre-Golgi intermediate compartment (IC) and its relationship with the ER and Golgi remain only partially understood. Here we report striking segregation of IC domains in polarized PC12 cells that develop neurite-like processes. Differentiation involves expansion of the IC and movement of Rab1-containing tubules to the growth cones of the neurites, whereas p58- and COPI-positive IC elements, like rough ER and Golgi, remain in the cell body. Exclusion of Rab1 effectors p115 and GM130 from the neurites further indicated that the centrifugal, Rab1-mediated pathway has functions that are not directly related to ER-Golgi trafficking. Disassembly of COPI coats did not affect this pathway, but resulted in missorting of p58 to the neurites. Live cell imaging showed that GFP-Rab1A-containing IC elements move bidirectionally both within the neurites and cell bodies, interconnecting different ER exit sites and the cis-Golgi region. Moreover, in nonpolarized cells GFP-Rab1A-positive tubules moved centrifugally toward the cell cortex. HMG-CoA reductase, the key enzyme of chole-sterol biosynthesis, colocalized with slowly-sedimenting, Rab1-enriched membranes when the IC subdomains were separated by velocity sedimentation. These results reveal a novel pathway directly connecting the IC with the cell periphery, and suggest that this Rab1-mediated pathway is linked to the dynamics of smooth ER.

{ddagger}Present address: Rockefeller University, Center for Studies in Physics and Biology, Living Matter Laboratory, Box 34, 1230 York Avenue, New York, NY 10021.

Address correspondence to: Jaakko Saraste (jaakko.saraste{at}biomed.uib.no)




This article has been cited by other articles:


Home page
 J. Neurosci.Home page
V. Reiterer, S. Maier, H. H. Sitte, A. Kriz, M. A. Ruegg, H.-P. Hauri, M. Freissmuth, and H. Farhan
Sec24- and ARFGAP1-Dependent Trafficking of GABA Transporter-1 Is a Prerequisite for Correct Axonal Targeting
J. Neurosci., November 19, 2008; 28(47): 12453 - 12464.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D. Carrel, J. Masson, S. Al Awabdh, C. B. Capra, Z. Lenkei, M. Hamon, M. B. Emerit, and M. Darmon
Targeting of the 5-HT1A Serotonin Receptor to Neuronal Dendrites Is Mediated by Yif1B
J. Neurosci., August 6, 2008; 28(32): 8063 - 8073.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
N. Rismanchi, C. Soderblom, J. Stadler, P.-P. Zhu, and C. Blackstone
Atlastin GTPases are required for Golgi apparatus and ER morphogenesis
Hum. Mol. Genet., June 1, 2008; 17(11): 1591 - 1604.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. Farhan, V. Reiterer, A. Kriz, H.-P. Hauri, M. Pavelka, H. H. Sitte, and M. Freissmuth
Signal-dependent export of GABA transporter 1 from the ER-Golgi intermediate compartment is specified by a C-terminal motif
J. Cell Sci., March 15, 2008; 121(6): 753 - 761.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
P. Monetta, I. Slavin, N. Romero, and C. Alvarez
Rab1b Interacts with GBF1 and Modulates both ARF1 Dynamics and COPI Association
Mol. Biol. Cell, July 1, 2007; 18(7): 2400 - 2410.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. Saraste and B. Goud
Functional Symmetry of Endomembranes
Mol. Biol. Cell, April 1, 2007; 18(4): 1430 - 1436.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. Yu, A. Satoh, M. Pypaert, K. Mullen, J. C. Hay, and S. Ferro-Novick
mBet3p is required for homotypic COPII vesicle tethering in mammalian cells
J. Cell Biol., July 31, 2006; 174(3): 359 - 368.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2006 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.